Identification of the Oxa-48 Gene Coding For Carbapenem Resistance in Klebsiella pneumoniae and Escherichia Coli at Dr. Muhammad Hoesin Central General Hospital, Palembang

Анотація

OXA-48 is a class D β-lactamase which is not inhibited by clavulanic acid, tazobactam and sulbactam, because their activity may be inhibited in vitro by NaCl. Some b-lactamase enzymes hydrolyze carbapenem and are therefore defined as hydrolysis of class D carbapenem b-lactamase. Carbapenem-resistant Klebsiella pneumoniae isolates were found in Istanbul, Turkey, in 2001. OXA-48 produces isolates that have the ability to fight drugs and cause very beneficial resistance to β-lactams, including widely available cephalosporins, cephamycins, and monobactone and carbapenem. The OXA-48 gene is found on plasmids. Antibiotic resistance is a phenomenon taken from the discovery of antibiotics where there is a very good adaptability for bacteria which are treated with various antibiotics. It has bacteria that have the ability to mutate as a protection for survival. This study is a case study involving the OXA gene as an encoder for carbapenem in Escherichia coli and Klebsiella pneumoniae using the Polymerase Chain Reaction (PCR) method to use genotypes used in hospitals that can be used in Dr. RSUP. Mohammad Hoesin Palembang. Bacterial isolates derived from patients with Klebsiella pneumoniae and Escherichia coli infections in the period September-November 2017 were identified using Vitek 2 Compact. bla_OXA-48 gene was detected by PCR, followed by visualization through electrophoresis. The detection results were then analyzed by comparing the pattern of antibiotic resistance. From 24 samples tested only 1 (4.7%) positive samples were obtained from the bacterium Klebsiellapneumoniae and no positive gene was found in Escherichia coli. This study identified 1 (4.2%) samples that had a positive OXA-48 gene while 23 (95.8%) samples had a negative OXA-48 gene.

DOI: 10.5281/zenodo.4382206