Development of electro-chemical sensor for determination of molybdenum (vi) contain in biological fluids

Authors

  • Катерина Вячеславівна Маторіна Oles Honchar Dnipropetrovsk National University Gagarina 72, Dnipropetrovsk, Ukraine, 49010, Ukraine

DOI:

https://doi.org/10.15587/1729-4061.2015.42821

Keywords:

potentiometry, sensor, molybdenum, triple metal-polymeric complex, biological fluid, urine, blood

Abstract

Theelectro-chemicalsensorfordeterminationofmolybdenum (VI) concentrationwiththeusingofthetriplemetal – polymeric complex bromopyrogallol red – polyvinylpyrrolidone - molybdenum (VI) as electrode – active substance of film plasticized membrane had been elaborated. The electrode-analytical characteristics of constructed sensor had been fixed: dynamic range (2-5 рС); the slope of electrode function - 27 mV/pC detection limit Сmin= 8·10-8 mol/l; the optimal range of pH sensor operation is 6,0 – 8,0; conditioning time – 48 h; response time – 1-2 minutes; lifetime – 12 months. The effect of the inorganic electrolytes presence in internal and external solutions on electrode – analytical characteristics of proposed sensor had been determinated. The selectivity coefficients (Кsel) of made sensor on relationships for inorganic (Pb2+; Ni2+; Cu2+; Cd2+; Zn2+; Ba2+; Fe3+; Bi3+; PO43- (1,0·10-3); Mg2+; Be2+; NO3-; SO42- (3,2·10-3); Cl- (5,0·10-3); K+, Mn2+, Br-, I- (1,0·10-2); Al3+ (2,2·10-2) Na+ (3,3·10-2)) and organic components (citric acid (1,0·10-3); thiourea (1,0·10-2); urea; oxalic acid; succinic acid; glucose (3,0·10-2)) ofbiological fluid were determinated by method of separated solutions. The determination of molybdenum (VI) contain in whole blood (sr=5,9 %) and urine (sr=5,9 %) samples was conducted by method of additions. The correctness of the obtained results was tested by alternative spectrophotometric technique. The reproducibility of proposed ionometric and alternative spectrophotometric techniques for Cochran's Q – test (Gexper < Gtabl) is the same. The proposed sensor can be used in clinical analyze of biological fluids, in particular in the whole bloodand urine samples of patients.

Author Biography

Катерина Вячеславівна Маторіна, Oles Honchar Dnipropetrovsk National University Gagarina 72, Dnipropetrovsk, Ukraine, 49010

Associated Professor, Candidate of Chemical Sciences

The department of Analytical Chemistry

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Published

2015-06-17

How to Cite

Маторіна, К. В. (2015). Development of electro-chemical sensor for determination of molybdenum (vi) contain in biological fluids. Eastern-European Journal of Enterprise Technologies, 3(6(75), 25–31. https://doi.org/10.15587/1729-4061.2015.42821

Issue

Vol. 3 No. 6(75) (2015): Technology organic and inorganic substances. Ecology

Section

Technology organic and inorganic substances

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Copyright (c) 2015 Катерина Вячеславівна Маторіна

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