EVALUATION OF STEVIA REBAUDIANA EFFECT ON LACTOBACILLUS PLANTARUM GROWTH: AN IN VITRO STUDY
DOI:
https://doi.org/10.32461/2226-3209.3.2018.171939Keywords:
Caries, Stevia Rebaudiana, Lactobacillus Plantarum.Abstract
Abstract. One way to control dental caries is the use of antibacterial mouthwash. The use of medicinal plants is highly considered, because of side effects and antibacterial resistance of the chemical substances. The aim of this study is to evaluate the antibacterial effects of Stevia Rebaudiana extract on Lactobacillus Plantarum. In this experimental invitro study, the extract of Stevia Rebaudiana was prepared. The indices of antibacterial activity were; minimum inhibitory concentration (MIC), maximum bacterial concentration (MBC) and the zone of growth inhibition of Stevia Rebaudiana against Lactobacillus Plantarum. These criteria were determined by broth macrodilution and agar well diffusion methods. The results was assessed by SPSS20 and one-way ANOVA (=0.05). Lactobacillus Plantarum showed the most sensitivity to Stevia Rebaudiana extract (p-Value=0.02). MIC and MBC for Stevia was 6.71 μg/ml and 26.87 μg/ml. The more the concentration of Stevia, the greater zone diameter of growth inhibition of bacteria (p-value=0.0). Maximum diameter of growth inhibition was measured for 880 μg/ml of Stevia. Assessment of zone of inhibition in 24 and 48 hours showed no significant difference (p-Value=0.7). The results of this study show that this plant has antibacterial effect, and can be used as a drug to prevent and control the growth of Lactobacillus Plantarum.
Keywords: Caries, Stevia Rebaudiana, Lactobacillus Plantarum.
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