Luminescent nanosized cell markers on the basis of llantanum phosfate

Authors

  • Олександр Володимирович Шаповал Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013, Ukraine
  • Олеся Степанівна Мягкота Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013, Ukraine
  • Ростислав Русланович Панчук Institute of Cell Biology NAS of Ukraine 14/16Drahomanov St., Lviv, Ukraine, 79005, Ukraine
  • Віталій Володимирович Вістовський Ivan Franko National University of Lviv 8 Kyrylo and Methodiy St., Lviv, Ukraine, 79005, Ukraine
  • Наталія Євгенівна Мітіна Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013, Ukraine
  • Анатолій Степанович Волошиновський Ivan Franko National University of Lviv 8 Kyrylo and Methodiy St., Lviv, Ukraine, 79005, Ukraine
  • Ростислав Степанович Стойка Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov St., Lviv, Ukraine, 79005, Ukraine
  • Олександр Сергійович Заіченко Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013, Ukraine

DOI:

https://doi.org/10.15587/1729-4061.2013.18929

Keywords:

luminescent nanoparticles, grafting polymerization, cell markers, functional oligoperoxides

Abstract

Luminescent properties of Lanthanum phosphate nanoparticles, doped with Europium cations with functional oligoperoxide shell were investigated. Water- and organic dispersion polymerization of a mixture of polar functional monomers, initiated by peroxide groups localized on the surface of LaPO4 • Eu3+ were carried out. Kinetic regularities of polymerization were investigated and the parameters of control were established. Grafting of functional polymer chains by water- and organic dispersion polymerization provides nanoparticles affinity to specific environments and materials, reactivity, biological tolerance and / or the possibility of binding to substrates of different nature for use in creating of luminescent biosensors and cell markers. Nanoparticles LaPO4 • Eu3+ with grafted poly(N-vinylpirrolidone - glycidyl methacrylate) polymeric shell were studied as analytical reagents for melanoma cancer cells labeling. When performing the study, the optimal concentrations of nanoparticles for cell labeling were determined.

Author Biographies

Олександр Володимирович Шаповал, Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013

Ph.D., Researcher

Department of Radioelectronic Appliances and Systems

Олеся Степанівна Мягкота, Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013

Ph.D. Student

Department of Organic Chemistry

Ростислав Русланович Панчук, Institute of Cell Biology NAS of Ukraine 14/16Drahomanov St., Lviv, Ukraine, 79005

Ph.D., Researcher

Department of Regulation of Cell Proliferation and Apoptosis

Віталій Володимирович Вістовський, Ivan Franko National University of Lviv 8 Kyrylo and Methodiy St., Lviv, Ukraine, 79005

Ph.D., Assoc. Professor

Department of Experimental Physics

Наталія Євгенівна Мітіна, Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013

Ph.D., senior resercher

Department of Technology of Organic Products

Анатолій Степанович Волошиновський, Ivan Franko National University of Lviv 8 Kyrylo and Methodiy St., Lviv, Ukraine, 79005

Dr. Sci., Professor

Department of Experimental Physics

Ростислав Степанович Стойка, Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov St., Lviv, Ukraine, 79005

Dr. Sci.,Professor

Department of Regulation of Cell Proliferation and Apoptosis

Олександр Сергійович Заіченко, Lviv Polytechnic National University 12 Stepan Bandera St., Lviv, Ukraine, 79013

Ph.D., Leading researcher

Department of Organic Chemistry

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Published

2013-12-17

How to Cite

Шаповал, О. В., Мягкота, О. С., Панчук, Р. Р., Вістовський, В. В., Мітіна, Н. Є., Волошиновський, А. С., Стойка, Р. С., & Заіченко, О. С. (2013). Luminescent nanosized cell markers on the basis of llantanum phosfate. Eastern-European Journal of Enterprise Technologies, 6(6(66), 32–35. https://doi.org/10.15587/1729-4061.2013.18929

Issue

Vol. 6 No. 6(66) (2013): Technology of organic and inorganic substanse

Section

Technology organic and inorganic substances

License

Copyright (c) 2014 Олександр Володимирович Шаповал, Олеся Степанівна Мягкота, Ростислав Русланович Панчук, Віталій Володимирович Вістовський, Наталія Євгенівна Мітіна, Анатолій Степанович Волошиновський, Ростислав Степанович Стойка, Олександр Сергійович Заіченко

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