Experimental substantiation of the application of plant extracts and enzymes to obtain safe raw materials for whole grain bread technology





enzyme preparations, whole grain bread, sea buckthorn, osehip and barberry extracts


Currently, ensuring the high quality and safety of food is the most important condition for a rational diet, maintaining health, mental and physical performance and supporting the body's defense systems. The modern environmental situation is one of the conditioning factors of the nutritional status of the population. It is known that more than 70 % of the total amount of pollutants entering the human body falls on food.

The purpose of this work is to improve the safety and quality of raw materials and grain products for whole-grain bread technology using enzyme preparations and extracts of plant origin during grain defrosting.

Reasonable effective doses of enzyme preparations have been determined. Cellolux was introduced at a dose of 0.03–0.08 % by weight of grain solids. To reduce the content of toxic elements in the grain, 0.05 % of the mass of grain solids was chosen, the optimal duration of defrosting is 6 and 12 hours, but depending on the efficiency of the time ‒ 6 hours. The grain was soaked at a temperature of 23 °C (room temperature).

Extracts of leaves and stems of rosehip, sea buckthorn and barberry were used as plant material. The use of these plant raw materials is explained by the fact that their leaves and bran extracts contain polyphenols with antioxidant, antimicrobial and carcinogenic properties. For disinfection and purification of grain from heavy metals, a content of 0.05 % of the grain weight was selected.

The effect of plant extracts on the microbiological contamination of grain during soaking and in preparation for the production of grain bread and preventing microbial spoilage of bakery products during storage has been studied. And extracts of rosehip leaves and sea buckthorn stalk have a pronounced antimicrobial effect against fungi of the genus Pénicillium. That will make it possible to exclude diseases of bread and improve its rheology

Author Biographies

Indira Kurmanbayeva, Almaty Technological University

Doctor of Philosophy

Department of Food Safety and Quality

Zhanar Nabiyeva, Almaty Technological University

PhD, Director of Research Institute

Research Institute of Food Safety

Albena Stoyanova, University of Food Technologies


Department of Tobacco, Sugar, Vegetable and Essential Oils Technology

Ainur Zheldybayeva, Almaty Technological University

Doctor of Philosophy, Associate Professor

Department of Food Safety and Quality

Dinara Tlevlessova, Almaty Technological University

Doctor of Philosophy, Associate Professor

Department of Food Technology


  1. Ibraimova, S., Uazhanova, R., Mardar, M., Serikbaeva, A., Tkachenko, N., Zhygunov, D. (2020). Development of recipe composition of bread with the inclusion of juniper using mathematical modeling and assessment of its quality. Eastern-European Journal of Enterprise Technologies, 6 (11 (108)), 6–16. doi: https://doi.org/10.15587/1729-4061.2020.219020
  2. Nuttall, J. G., O’Leary, G. J., Panozzo, J. F., Walker, C. K., Barlow, K. M., Fitzgerald, G. J. (2017). Models of grain quality in wheat – A review. Field Crops Research, 202, 136–145. doi: https://doi.org/10.1016/j.fcr.2015.12.011
  3. Nađpal, J. D., Lesjak, M. M., Šibul, F. S., Anačkov, G. T., Četojević-Simin, D. D., Mimica-Dukić, N. M., Beara, I. N. (2016). Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds. Food Chemistry, 192, 907–914. doi: https://doi.org/10.1016/j.foodchem.2015.07.089
  4. Williams, P. (2005). Consumer Understanding and Use of Health Claims for Foods. Nutrition Reviews, 63 (7), 256–264. doi: https://doi.org/10.1111/j.1753-4887.2005.tb00382.x
  5. Thielecke, F., Nugent, A. (2018). Contaminants in Grain – A Major Risk for Whole Grain Safety? Nutrients, 10 (9), 1213. doi: https://doi.org/10.3390/nu10091213
  6. Poutanen, K. (1997). Enzymes: An important tool in the improvement of the quality of cereal foods. Trends in Food Science & Technology, 8 (9), 300–306. doi: https://doi.org/10.1016/s0924-2244(97)01063-7
  7. Vaičiulytė-Funk, L., Žvirdauskienė, R., Šalomskienė, J., Šarkinas, A. (2015). The effect of wheat bread contamination by the Bacillus genus bacteria on the quality and safety of bread. Zemdirbyste-Agriculture, 102 (3), 351–358. doi: https://doi.org/10.13080/z-a.2015.102.045
  8. Ghendov-Mosanu, A., Cristea, E., Patras, A., Sturza, R., Padureanu, S., Deseatnicova, O. et al. (2020). Potential Application of Hippophae Rhamnoides in Wheat Bread Production. Molecules, 25 (6), 1272. doi: https://doi.org/10.3390/molecules25061272
  9. Ayati, Z., Amiri, M. S., Ramezani, M., Delshad, E., Sahebkar, A., Emami, S. A. (2019). Phytochemistry, Traditional Uses and Pharmacological Profile of Rose Hip: A Review. Current Pharmaceutical Design, 24 (35), 4101–4124. doi: https://doi.org/10.2174/1381612824666181010151849
  10. Kurmanbaeva, I. N., Nabieva, Zh. S. (2019). Bөrіқaraқattyң biokhimiyalyқ құramy zhәne emdіk қasietterі. Semey қalasynyң Shәkәrіm atyndaғy memlekettіk universitetіnің khabarshysy, 4 (88).
  11. Nabieva, Zh. S., Kurmanbaeva, I. N., Shukesheva, S. E., Zhayyrbaeva, M. B. (2020). Sposoby povysheniya mikrobiologicheskoy bezopasnosti syr'ya i gotovykh izdeliy iz tsel'nykh zeren. Mezhdunarodnaya nauchno-prakticheskaya konferentsiya «Zernovaya otrasl': Sostoyanie i perspektivy razvitiya». Almaty.
  12. Kurmanbaeva, I. N., Nabieva, Zh. S., Zhel'dybaeva, A. A. (2022). Fermenttіk preparattar zhәne olardyң astyқty өңdeudegі rөlі. Vestnik ATU, 1 (135), 53–59.
  13. Tan, B., Wu, N.-N., Zhai, X.-T. (2020). Solutions for whole grain food development. Nutrition Reviews, 78, 61–68. doi: https://doi.org/10.1093/nutrit/nuz068
  14. Oliinyk, S., Samokhvalova, O., Zaparenko, A., Shidakova-Kamenyuka, E., Chekanov, M. (2016). Research into the impact of enzyme preparations on the processes of grain dough fermentation and bread quality. Eastern-European Journal of Enterprise Technologies, 3 (11 (81)), 46–53. doi: https://doi.org/10.15587/1729-4061.2016.70984
  15. Pshenyshniuk, G. F., Makarova, O. V., Ivanova, G. S. (2010). Innovatsiyni zahody pidvyshtschennia yakosti zernovogo khliba. Kharchova nauka i technologiya, 1, 73–77.
  16. Nasrullayeva, G. М., Yusifova, M. R. (2021). The influence of enzyme preparations on general indicators of grain. 70th International Scientific Conference on Economic and Social Development. Baku, 146–150.
  17. Ognean, M., Ognean, C. F., Bucur, A. (2011). Rheological Effects of Some Xylanase on Doughs from High and Low Extraction Flours. Procedia Food Science, 1, 308–314. doi: https://doi.org/10.1016/j.profoo.2011.09.048
  18. Shah, A. R., Shah, R. K., Madamwar, D. (2006). Improvement of the quality of whole wheat bread by supplementation of xylanase from Aspergillus foetidus. Bioresource Technology, 97 (16), 2047–2053. doi: https://doi.org/10.1016/j.biortech.2005.10.006
  19. Khmeleva, E., Berezina, N., Khmelev, A., Kunitsyna, T., Makarova, N. (2020). Aspects of environmental safety improving of whole grain bakery products. IOP Conference Series: Earth and Environmental Science, 421 (3), 032062. doi: https://doi.org/10.1088/1755-1315/421/3/032062
  20. Renzetti, S., Rosell, C. M. (2016). Role of enzymes in improving the functionality of proteins in non-wheat dough systems. Journal of Cereal Science, 67, 35–45. doi: https://doi.org/10.1016/j.jcs.2015.09.008
  21. Primo-Martin, C., Martinez-Anaya, M. A. (2003). Influence of Pentosanase and Oxidases on Water-extractable Pentosans during a Straight Breadmaking Process. Journal of Food Science, 68 (1), 31–41. doi: https://doi.org/10.1111/j.1365-2621.2003.tb14110.x
  22. Kuznetsova, E., Cherepnina, L., Motyleva, S., Brindza, J. (2016). Redistribution of mineral elements in wheat grain when applying the complex enzyme preparations based on phytase. Potravinarstvo Slovak Journal of Food Sciences, 10 (1), 47–53. doi: https://doi.org/10.5219/413
  23. Michel, T., Destandau, E., Le Floch, G., Lucchesi, M. E., Elfakir, C. (2012). Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chemistry, 131 (3), 754–760. doi: https://doi.org/10.1016/j.foodchem.2011.09.029
  24. Upadhyay, N. K., Yogendra Kumar, M. S., Gupta, A. (2010). Antioxidant, cytoprotective and antibacterial effects of Sea buckthorn (Hippophae rhamnoides L.) leaves. Food and Chemical Toxicology, 48 (12), 3443–3448. doi: https://doi.org/10.1016/j.fct.2010.09.019
  25. Ouerghemmi, S., Sebei, H., Siracusa, L., Ruberto, G., Saija, A., Cimino, F., Cristani, M. (2016). Comparative study of phenolic composition and antioxidant activity of leaf extracts from three wild Rosa species grown in different Tunisia regions: Rosa canina L., Rosa moschata Herrm. and Rosa sempervirens L. Industrial Crops and Products, 94, 167–177. doi: https://doi.org/10.1016/j.indcrop.2016.08.019
  26. Baimagambetova, K. K., Abugaliev, S. G. (2012). Prospects for variety selection of spring wheat Open Company «the Kazakh scientific research institute of agriculture and plant growing» JSC «КаzАgroInnovation» MSH RK. KazNU Bulletin. Biology series, 3 (55).
  27. Rimareva, L. V., Serba, E. M., Sokolova, E. N., Borshcheva, Yu. A., Ignatova, N. I. (2017). Enzyme preparations and biocatalytic processes in the food industry. Voprosy pitaniia [Problems of Nutrition], 86 (5), 62–74. doi: https://doi.org/10.24411/0042-8833-2017-00078
  28. Nabiyeva, Z., Zhexenbay, N., Iskakova, G., Kizatova, M., Akhmetsadykova, S. (2021). Devising technology for dairy products involvinglow-esterified pectin products. Eastern-European Journal of Enterprise Technologies, 3 (11 (111)), 17–27. doi: https://doi.org/10.15587/1729-4061.2021.233821
  29. Gartovannaya, E., Ermolaeva, A. (2021). Prospects of Using Whole Grain Flour from Recognized Selection Wheat Varieties of the Far Eastern State Agrarian University in Food Technologies. Lecture Notes in Networks and Systems, 357–365. doi: https://doi.org/10.1007/978-3-030-91402-8_41
  30. Capita, R., Prieto, M., Alonso-Calleja, C. (2004). Sampling Methods for Microbiological Analysis of Red Meat and Poultry Carcasses. Journal of Food Protection, 67 (6), 1303–1308. doi: https://doi.org/10.4315/0362-028x-67.6.1303
  31. Wagner, A. O., Markt, R., Mutschlechner, M., Lackner, N., Prem, E. M., Praeg, N., Illmer, P. (2019). Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions. Journal of Visualized Experiments, 150. doi: https://doi.org/10.3791/60155
  32. Fodor-Csorba, K. (1992). Chromatographic methods for the determination of pesticides in foods. Journal of Chromatography A, 624 (1-2), 353–367. doi: https://doi.org/10.1016/0021-9673(92)85688-p
  33. M-04-41-2005: Metodika vypolneniya izmereniy massovoy doli svobodnyh form vodorastvorimyh vitaminov v probah premiksov, vitaminnyh dobavok, kontsentratov i smesey metodom kapillyarnogo elektroforeza s ispol'zovaniem sistemy kapillyarnogo elektroforeza «Kapel'-105». Sankt-Peterburg: OOO «Lyumeks», 36.
  34. Alzahrani, Y., Rady, M. M. (2019). Compared to antioxidants and polyamines, the role of maize grain-derived organic biostimulants in improving cadmium tolerance in wheat plants. Ecotoxicology and Environmental Safety, 182, 109378. doi: https://doi.org/10.1016/j.ecoenv.2019.109378
  35. Koryachkina, S. Ya. (2002). Nekotorye aspekty sovershenstvovaniya tekhnologii khleba iz tselogo zerna. Uspekhi sovremennogo estestvoznaniya, 3, 56–61.
  36. da Cruz Cabral, L., Fernández Pinto, V., Patriarca, A. (2013). Application of plant derived compounds to control fungal spoilage and mycotoxin production in foods. International Journal of Food Microbiology, 166 (1), 1–14. doi: https://doi.org/10.1016/j.ijfoodmicro.2013.05.026
  37. Saljoqi, A. U. R., Afridi, M. K., Khan, S. A. et al. (2006). Effects of six plant extracts on rice weevil Sitophilus oryzae L. in the stored wheat grains. Journal of Agricultural and Biological Science, 1 (4). Available at: https://www.researchgate.net/publication/253884645
  38. Mohamed Baka, Z. A. (2014). Plant Extract Control of the Fungi Associated with Different Egyptian Wheat Cultivars Grains. Journal of Plant Protection Research, 54 (3), 231–237. doi: https://doi.org/10.2478/jppr-2014-0035
Experimental substantiation of the application of plant extracts and enzymes to obtain safe raw materials for whole grain bread technology




How to Cite

Kurmanbayeva, I., Nabiyeva, Z., Stoyanova, A., Zheldybayeva, A., & Tlevlessova, D. (2022). Experimental substantiation of the application of plant extracts and enzymes to obtain safe raw materials for whole grain bread technology. Eastern-European Journal of Enterprise Technologies, 6(11 (120), 89–98. https://doi.org/10.15587/1729-4061.2022.267230



Technology and Equipment of Food Production