Determining patterns of lactose hydrolysis in liquid concentrates of demineralized whey

Authors

DOI:

https://doi.org/10.15587/1729-4061.2024.318337

Keywords:

lactose hydrolysis, enzyme, acidophilic bacillus, whey concentrate, galactose, glucose

Abstract

The object of this study was the process of milk sugar hydrolysis in liquid whey concentrates. The problem solved was the intensification of the lactose hydrolysis reaction in liquid whey concentrates through the use of a combination of leavening and enzyme preparations. Patterns in the lactose hydrolysis process in liquid concentrates of demineralized whey were studied.

It was established that the use of an enzyme preparation for liquid whey concentrates does not make it possible to achieve a degree of lactose hydrolysis higher than 75–77 % within 10 h. The simultaneous use of enzyme and leavening preparations for 6 hours ensures the conversion of more than 95 % of lactose for concentrates with a mass fraction of dry substances of 10–30 % and more than 90 % for a 40 % concentrate. The rational duration of lactose hydrolysis in demineralized whey concentrates for the combination of preparations is 6 hours for 10 and 20 % concentrates, and 8 h for 30 and 40 % concentrates, which enables lactose hydrolysis at the level of 96.8–100 %. The rheological properties of concentrates with a solids content of 30 and 40 % indicate that these systems have a high ability to restore the structure.

The dynamics of monosaccharide formation during hydrolysis are similar for 10 and 20 % concentrates, in which galactose slightly predominates the systems. Data on the increase in glucose content in fermented 30 and 40 % concentrates contradict the known data regarding the consumption of glucose by the acidophilic bacillus and its conversion to galactose. This may indicate suppression of the activity of the acidophilic bacillus under conditions of increased osmotic pressure in the concentrates.

The results of the work could be used in the technology of whey ice cream, as well as dairy products that require adjustment of the chemical composition, primarily in terms of protein and lactose content

Author Biographies

Artur Mykhalevych, National University of Food Technologies

PhD Student

Department of Technology Мilk and Dairy Products

Lydmila Moiseyeva, Institute of Food Resources of the National Academy of Sciences of Ukraine

PhD

Department of Dairy Products and Baby Food

Galyna Polishchuk, National University of Food Technologies

Doctor of Technical Sciences, Professor

Department of Technology Мilk and Dairy Products

Uliana Bandura, National University of Food Technologies

PhD, Associate Professor

Department of Technology Мilk and Dairy Products

References

  1. Henriques, M. H. F., Gomes, D. M. G. S., Pereira, C. J. D., Gil, M. H. M. (2012). Effects of Liquid Whey Protein Concentrate on Functional and Sensorial Properties of Set Yogurts and Fresh Cheese. Food and Bioprocess Technology, 6 (4), 952–963. https://doi.org/10.1007/s11947-012-0778-9
  2. Henriques, M. H. F., Gomes, D. M. G. S., Borges, A. R., Pereira, C. J. D. (2020). Liquid whey protein concentrates as primary raw material for acid dairy gels. Food Science and Technology, 40 (2), 361–369. https://doi.org/10.1590/fst.43218
  3. Pereira, C., Henriques, M., Gomes, D., Gomez-Zavaglia, A., de Antoni, G. (2015). Novel Functional Whey-Based Drinks with Great Potential in the Dairy Industry. Food Technology and Biotechnology, 53. https://doi.org/10.17113/ftb.53.03.15.4043
  4. Mykhalevych, A., Kostenko, O., Polishchuk, G., Bandura, U. (2022). Application of milk protein concentrates in preparation of reduced fat sour cream. Ukrainian Food Journal, 11 (3), 429–447. https://doi.org/10.24263/2304-974x-2022-11-3-8
  5. Polishchuk, G., Sharakhmatova, T., Shevchenko, I., Manduk, O., Mykhalevych, A., Pukhlyak, A. (2023). Scientific substantiation of cream heating duration in the technology of sour cream, enriched with protein. Food Science and Technology, 17 (3). https://doi.org/10.15673/fst.v17i3.2657
  6. Mykhalevych, A., Buniowska-Olejnik, M., Polishchuk, G., Puchalski, C., Kamińska-Dwórznicka, A., Berthold-Pluta, A. (2024). The Influence of Whey Protein Isolate on the Quality Indicators of Acidophilic Ice Cream Based on Liquid Concentrates of Demineralized Whey. Foods, 13 (1), 170. https://doi.org/10.3390/foods13010170
  7. Shevchenko, O., Mykhalevych, A., Polischuk, G., Buniowska-Olejnik, M., Bass, O., Bandura, U. (2022). Technological functions of hydrolyzed whey concentrate in ice cream. Ukrainian Food Journal, 11 (4), 498–517. https://doi.org/10.24263/2304-974x-2022-11-4-3
  8. Henriques, M., Gomes, D., Pereira, C. (2017). Liquid Whey Protein Concentrates Produced by Ultrafiltration as Primary Raw Materials for Thermal Dairy Gels. Food Technology and Biotechnology, 55 (4). https://doi.org/10.17113/ftb.55.04.17.5248
  9. Bondar, S., Trubnikova, A., Chabanova, O. (2018). Investigation of membrane process for the lactose extract from buttermilk con-centrates. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 20 (85), 62–69. https://doi.org/10.15421/nvlvet8512
  10. Matijević, B., Lisak, K., Božanić, R., Tratnik, L. (2011). Impact of enzymatic hydrolyzed lactose on fermentation and growth of probiotic bacteria in whey. Mljekarstvo: časopis za unaprjeđenje proizvodnje i prerade mlijeka, 61 (2), 154–160.
  11. Vasudha, M., Prashantkumar, C., Bellurkar, M., Kaveeshwar, V., Gayathri, D. (2023). Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization. Biomedical Reports, 18 (3). https://doi.org/10.3892/br.2023.1605
  12. Romanchuk, I., Moiseieva, L., Minorova, A., Rudakova, T., Krushelnytska, N. (2023). Study of lactase activity of starter cultures in milk mixtures based on secondary milk raw materials. Food Resources, 11 (20), 119–129. https://doi.org/10.31073/foodresources2023-20-12
  13. Trubnikova, A., Sharakhmatova, T., Mamintova, K., Tsupra, O. (2018). Biotechnological aspects of a yogurt base from buttermilk for the production of low-lactose ice cream. Bulletin of the National Technical University «KhPI» Series: New Solutions in Modern Technologies, 9 (1285), 243–255. https://doi.org/10.20998/2413-4295.2018.09.35
  14. Dickson, R. C., Dickson, L. R., Markin, J. S. (1979). Purification and properties of an inducible beta-galactosidase isolated from the yeast Kluyveromyces lactis. Journal of Bacteriology, 137 (1), 51–61. https://doi.org/10.1128/jb.137.1.51-61.1979
  15. Shi, X., Wu, D., Xu, Y., Yu, X. (2022). Engineering the optimum pH of β-galactosidase from Aspergillus oryzae for efficient hydrolysis of lactose. Journal of Dairy Science, 105 (6), 4772–4782. https://doi.org/10.3168/jds.2021-21760
  16. Romanchuk, I., Minorova, A., Rudakova, T., Moiseeva, L. (2020). Regularities of lactose hydrolysis in dairy raw materials. Food Resources, 8 (14), 165–174. Available at: https://iprjournal.kyiv.ua/index.php/pr/article/view/55
  17. Krasulia, O., Hrek, O. (2013). Hidroliz laktozy molochnoi syrovatky z kharchovymy voloknamy. Prodovolcha industriia APK, 1, 38–40. Available at: http://nbuv.gov.ua/UJRN/Piapk_2013_1_12
  18. Weese, S. J., Gosnell, K., West, P., Gropper, S. S. (2003). Galactose content of baby food meats: Considerations for infants with galactosemia. Journal of the American Dietetic Association, 103 (3), 373–375. https://doi.org/10.1053/jada.2003.50043
  19. Dominici, S., Marescotti, F., Sanmartin, C., Macaluso, M., Taglieri, I., Venturi, F. et al. (2022). Lactose: Characteristics, Food and Drug-Related Applications, and Its Possible Substitutions in Meeting the Needs of People with Lactose Intolerance. Foods, 11 (10), 1486. https://doi.org/10.3390/foods11101486
  20. Mykhalevych, A., Polishchuk, G., Bandura, U., Osmak, T., Bass, O. (2024). Determining the influence of plant-based proteins on the characteristics of dairy ice cream. Technology and Equipment of Food Production, 4 (11 (130)), 6–15. https://doi.org/10.15587/1729-4061.2024.308635
  21. Dutra Rosolen, M., Gennari, A., Volpato, G., Volken de Souza, C. F. (2015). Lactose Hydrolysis in Milk and Dairy Whey Using Microbial β-Galactosidases. Enzyme Research, 2015, 1–7. https://doi.org/10.1155/2015/806240
  22. Horner, T. W., Dunn, M. L., Eggett, D. L., Ogden, L. V. (2011). β-Galactosidase activity of commercial lactase samples in raw and pasteurized milk at refrigerated temperatures. Journal of Dairy Science, 94 (7), 3242–3249. https://doi.org/10.3168/jds.2010-3742
  23. Akgül, F. B., Demirhan, E., Özbek, B. (2012). A Modelling study on skimmed milk lactose hydrolysis and β‐galactosidase stability using three reactor types. International Journal of Dairy Technology, 65 (2), 217–231. https://doi.org/10.1111/j.1471-0307.2012.00828.x
  24. Antunes, A. E. C., Silva e Alves, A. T., Gallina, D. A., Trento, F. K. H. S., Zacarchenco, P. B., Van Dender, A. G. F. et al. (2014). Development and shelf-life determination of pasteurized, microfiltered, lactose hydrolyzed skim milk. Journal of Dairy Science, 97 (9), 5337–5344. https://doi.org/10.3168/jds.2014-8020
  25. Hourigan, J. A. (1984). Nutritional implications of lactose. Australian Journal of Dairy Technology, 39, 114–120.
  26. Jurado, E., Camacho, F., Luzón, G., Vicaria, J. M. (2004). Kinetic models of activity for β-galactosidases: influence of pH, ionic concentration and temperature. Enzyme and Microbial Technology, 34 (1), 33–40. https://doi.org/10.1016/j.enzmictec.2003.07.004
  27. Wang, D., Sakakibara, M. (1997). Lactose hydrolysis and β-galactosidase activity in sonicated fermentation with Lactobacillus strains. Ultrasonics Sonochemistry, 4 (3), 255–261. https://doi.org/10.1016/s1350-4177(96)00042-9
  28. Alm, L. (1982). Effect of Fermentation on Lactose, Glucose, and Galactose Content in Milk and Suitability of Fermented Milk Products for Lactose Intolerant Individuals. Journal of Dairy Science, 65 (3), 346–352. https://doi.org/10.3168/jds.s0022-0302(82)82198-x
  29. Shwe, T., Pratchayasakul, W., Chattipakorn, N., Chattipakorn, S. C. (2018). Role of D-galactose-induced brain aging and its potential used for therapeutic interventions. Experimental Gerontology, 101, 13–36. https://doi.org/10.1016/j.exger.2017.10.029
  30. Puangmanee, S., Hayakawa, S., Sun, Y., Ogawa, M. (2008). Application of Whey Protein Isolate Glycated with Rare Sugars to Ice Cream. Food Science and Technology Research, 14 (5), 457–466. https://doi.org/10.3136/fstr.14.457
Determining patterns of lactose hydrolysis in liquid concentrates of demineralized whey

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Published

2024-12-27

How to Cite

Mykhalevych, A., Moiseyeva, L., Polishchuk, G., & Bandura, U. (2024). Determining patterns of lactose hydrolysis in liquid concentrates of demineralized whey. Eastern-European Journal of Enterprise Technologies, 6(11 (132), 24–32. https://doi.org/10.15587/1729-4061.2024.318337

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Technology and Equipment of Food Production