Development of biotechnology of hydrogen sulfid removal from biogas using immobilization material based on phosphogypsum




biotechnology, hydrogen sulfide removal, biogas, phosphogypsum, immobilization of microorganisms, sulfur


The paper focuses on the possibility of using phosphogypsum in the biotechnology processes of gas purification. The biomass of Thiobacillus sp. was immobilized on the granulated material of support medium consisting of dihydrate phosphogypsum. Desulfurization resulted in forming of acidophilic microorganisms association that is able to oxidize hydrogen sulfide to form elemental sulfur in an acidic environment. The granulated loading was based on phosphogypsum and has the following advantages: it has low cost; it stimulates the development of needful ecological-trophic groups of microorganisms; it creates favorable conditions for the formation of biofilm on their surface; the contact surface extends with a gas stream; it is capable of regeneration; it is resistant to higher acidity; it has the protection function blocking toxic components; it increases the yield of elemental sulfur. Optimum conditions of biological system of hydrogen sulfide removal from biogas were determined.

Author Biographies

Єлізавета Юріївна Черниш, Sumy State University Str. Rymskogo-Korsakova 2, Sumy, Ukraine, 40007

Assistant, Candidate of technical science

The department of applied ecology

Леонід Дмитрович Пляцук, Sumy State University Str. Rymskogo-Korsakova 2, Sumy, Ukraine, 40007

Professor, Doctor of Technical Sciences, Head of the department

The department of applied ecology


  1. Geletukha, G. G., Kucheruk, P. P., Matveev, Yu. B. (2014). Prospects for production and use of biomethane in Ukraine. Analiticheskaya zapiska BAU, 11. Kiev: Bioenergeticheskaya assotsiatsiya Ukrainyi, 44.
  2. Piatnichko, A. I., Ivanov, J. V., Krushnevich, T. K. (2010). Optimization of water-amine solutions as absorbents a unit of biomethane extraction from biogas. Tehnicheskie gazyi, 3, 26–29.
  3. Idigenov, A. B., Filatov, M. I. (2013). Installation of the combined cleaning of biogas. Vestnik Saratovskogo gosudarstvennogo tehnicheskogo universiteta, 2 (71), 94–101.
  4. Ramirez, M., Gómez, J., Cantero, D. (2009). Removal of hydrogen sulphide by immobilized Thiobacillus thioparus in a biofilter packed with polyurethane foam. Bioresource Technology, 100 (21), 4989–4995. doi: 10.1016/j.biortech.2009.05.022
  5. Janssen, A. J., Ma, S. C., Lens, P., Lettinga, G. (1997). Performance of a sulfide oxidizing expanded bed reactor supplied with dissolved oxygen. Biotechnology and Bioengineering, 53 (1), 32–40. doi: 10.1002/(sici)1097-0290(19970105)53:1<32::aid-bit6>;2-#
  6. Ravichandra, P., Mugeraya, G., Gangagni Rao, A., Ramakrishna, M., Annapurna, J. (2007). Isolation of Thiobacillus sp from aerobic sludge of distillery and dairy effluent treatment plants and its sulfide oxidation activity at different concentrations. Journal of Environmental Biology, 28 (4), 819–823.
  7. Park Byoung-Gi, C., Shin, W. S., Chung, J. S. (2008). Simultaneous biofiltration of H2S, NH3 and toluene using an inorganic polymeric composite. Environmental Engineering Research, 13 (1), 19–27. doi: 10.4491/eer.2008.13.1.019
  8. Desulfurization technology THIOPAQ O&G. Information from the website of the enterprise «Paqell» (2014). Available at:
  9. Bakhareva, A. Yu., Yurchenko, V. A. (2013). Dangerous concentration of methane in gasiform emissions and methods of its reduction. Eastern-European Journal of Enterprise Technologies, 3/11(63), 8–11. Available at:
  10. Xіl'chevs'kij, V. K. (2007). Vidhody virobnitstva i spozhivannya ta yih vpliv na grunti I prirodnI vody [Waste production and consumption and their impact on soil and natural water]. Kiev: Vidavnicho-poligrafichniy tsentr "Kiyivskiy universitet", 152.
  11. Abdrakipov, A. R., Barahnina, V. B., Jagafarova, G. G. (2010). Phosphogypsum use at the oil-field sewage bioclearing. Aktualnyie ekologicheskie problemyi: Sbornik nauchnyih trudov V mezhdunarodnoy nauchno-prakticheskoy konferentsii, 4–6.
  12. Kaniskina, M. A., Terekhov, V. A., Semenova, T. A., Lisak, L. V. (2009). Influence of phosphogypsum on microorganisms soil-ground. Dokladyi po ekologicheskomu pochvovedeniyu, 1 (11), 62–78.
  13. Kaniskin, M. A., Terehova, V. A., Yakovlev, A. S. (2007). Control humate detoxification of phosphogypsum waste by bioassay methods. Ekologiya i promyishlennost, 8, 48–51.
  14. Belyuchenko, I. S. (2014). Complex composts and detoxication of agrolandscape systems. Nauchnyiy zhurnal KubGAU, 97 (03). Available at:



How to Cite

Черниш, Є. Ю., & Пляцук, Л. Д. (2015). Development of biotechnology of hydrogen sulfid removal from biogas using immobilization material based on phosphogypsum. Eastern-European Journal of Enterprise Technologies, 2(6(74), 28–34.



Technology organic and inorganic substances