COMPUTING EXPERIMENT OF ALBUMIN SEPARATION OF BONDED BILIRUBIN IN MICROFLUID CHIP
DOI:
https://doi.org/10.15587/1729-4061.2012.4526Keywords:
albumin, bilirubin, polymethylmethacrylate, separation, finite difference, capillary electrophoresis, microfluid chipAbstract
There is a research of the separation process of albumin and bilirubin, which migrate in the buffer solution in the direct separation channel of microfluid polymethylmethacrylate chip under different conditions (voltage of 1 kV and 2kV, the length of separation channel 2 and 5 cm, and pH 7). On the basis of the model of electrokinetic motion, realized by finite difference method, the nature of the distribution of these substances along the sample zone at the capillary electrophoresis was determined. It is shown that achievement of high-speed separation is possible by increasing of electric field voltage. It is noted that the fastest separation of bilrubin and albumin takes 8 s. This computing experiment is important when choosing the optimal parameters, mentioned above, before design and engineering, and experimental stages of production of the microfluid chip.References
- Rolinski B. Total Bilirubin Measurement by Photometry on a Blood Gas Analyzer: Potential for Use in Neonatal Testing at the Point of Care. Clinical Chemistry, 2001, vol. 47, pp. 1845-1847.
- Muzyka E.N., Rozhitskii N.N., Systems of Capillary Electrophoresis in Electrochemiluminescence Analysis. J of Anal Chemistry, 2010, vol. 65, pp.550–564.
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- Fung Y.S., Sun D. X. et al. Capillary electrophoresis for determination of free and albumin-bound bilirubin and the investigation of drug interaction with bilirubin-bound albumin. Electrophoresis, 2000, vol. 21, pp. 403-410.
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