Effect of lactic acid microorganisms on the content of nitrates in tomato in the process of pickling

Authors

DOI:

https://doi.org/10.15587/1729-4061.2018.120548

Keywords:

nitrates, vegetable produce, denitrification process, microbiological process, lactic acid microflora

Abstract

We established the content of nitrates in vegetables and studied special features in the course of microbiological and denitrification processes during pickling of tomatoes with a different content of nitrates. It was found that 15 % on average of the samples of vegetable produce, grown in open ground, contained the amount of nitrates that exceeded MPC (maximum permissible concentration). For vegetables grown in greenhouses, the number of samples with the nitrate content above the norm reached 25−30 %. It was discovered that in the process of pickling tomatoes with the amount of nitrates at 137±10 mg/kg (within MPC), there occurs an intensive growth of lactic acid microorganisms. As a result of the reproduction of lactic acid microflora, there happens a denitrification process with the amount of nitrates in pickled tomatoes reduced to 17±2 mg/kg. When pickling tomatoes with a nitrate content twice as large than MPC at 619±32 mg/kg, microbiological and denitrification processes slow down at the beginning. Then they are recovered with the amount of nitrates reduced to 134±5 mg/kg. When pickling tomatoes with the nitrate content five times larger than MPC (1,576±114 mg/kg), the pickling does not occur because of the inhibiting influence of nitrates on the growth of microflora.

Thus, the data obtained indicate that the tomatoes that contain nitrates within 600−700 mg/kg can be used for the preparation of pickled products. Vegetable produce containing nitrates in the amount above 1,500 mg/kg must not be used in pickling due to the inhibiting influence of nitrates on lactic acid microflora

Author Biographies

Mykola Kukhtyn, Ternopil Ivan Puluj National Technical University Ruska str., 56, Ternopil, Ukraine, 46001

Doctor of Veterinary Sciences, Professor

Department of Food Biotechnology and Chemistry 

Yulia Horiuk, State Agrarian and Engineering University in Podilya Shevchenka str., 13, Kamianets-Podilskyi, Ukraine, 32300

PhD, Senior Lecturer

Department of Infectious and Parasitic Diseases

Tetiana Yaroshenko, I. Horbachevsky Ternopyl State Medical University Voli sq., 1, Ternopil, Ukraine, 46001

PhD, Senior Lecturer

Department of Medical Biochemistry

Svitlana Laiter-Moskaliuk, State Agrarian and Engineering University in Podilya Shevchenka str., 13, Kamianets-Podilskyi, Ukraine, 32300

PhD, Senior Lecturer

Department of Microbiology, Pharmacology and Animal Hygiene

Viktoria Levytska, State Agrarian and Engineering University in Podilya Shevchenka str., 13, Kamianets-Podilskyi, Ukraine, 32300

PhD, Senior Lecturer

Department of Infectious and Parasitic Diseases

Antonina Reshetnyk, State Agrarian and Engineering University in Podilya Shevchenka str., 13, Kamianets-Podilskyi, Ukraine, 32300

PhD, Associate Professor

Department of Microbiology, Pharmacology and Animal Hygiene 

References

  1. Kharitonov, M. M., Lazareva, O. M., Lemishko, S. M. (2015). Environmental assessment of the variability of nitrate content in vegetable and fruit and berry crops in the Dnipropetrovsk region. Newsletter of the Poltava State Agrarian Academy, 3, 29–31.
  2. Hord, N. G., Tang, Y., Bryan, N. S. (2009). Food sources of nitrates and nitrites: the physiologic context for potential health benefits. American Journal of Clinical Nutrition, 90 (1), 1–10. doi: 10.3945/ajcn.2008.27131
  3. Merino, L., Örnemark, U., Toldrá, F. (2017). Analysis of Nitrite and Nitrate in Foods. Advances in Food and Nutrition Research, 65–107. doi: 10.1016/bs.afnr.2016.11.004
  4. Davydovskiy, A. G. (2009). Metabolicheskaya korrektsiya redoks-statusa eritrotsitov pri bakterial'noy endotoksemii i nitrit indutsirovannoy metgemoglobinemii. Zdorov'e i okruzhayushchaya sreda, 13, 317–323.
  5. Tociu, C., Marcu, E., Ciobotaru, I. E., Maria, C. (2016). Risk assessment of population exposure to nitrates/nitrites in groundwater: a case study approach. Ecoterra-Journal of Environmental Research and Protection, 13 (3), 39–45.
  6. Quijano, L., Yusà, V., Font, G., McAllister, C., Torres, C., Pardo, O. (2017). Risk assessment and monitoring programme of nitrates through vegetables in the Region of Valencia (Spain). Food and Chemical Toxicology, 100, 42–49. doi: 10.1016/j.fct.2016.12.010
  7. Iammarino, M., Di Taranto, A., Cristino, M. (2013). Endogenous levels of nitrites and nitrates in wide consumption foodstuffs: Results of five years of official controls and monitoring. Food Chemistry, 140 (4), 763–771. doi: 10.1016/j.foodchem.2012.10.094
  8. Hsu, J., Arcot, J., Alice Lee, N. (2009). Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary intake in Australians. Food Chemistry, 115 (1), 334–339. doi: 10.1016/j.foodchem.2008.11.081
  9. Song, X.-Z., Zhao, C.-X., Wang, X.-L., Li, J. (2009). Study of nitrate leaching and nitrogen fate under intensive vegetable production pattern in northern China. Comptes Rendus Biologies, 332 (4), 385–392. doi: 10.1016/j.crvi.2008.11.005
  10. Kraft, G. J., Stites, W. (2003). Nitrate impacts on groundwater from irrigated-vegetable systems in a humid north-central US sand plain. Agriculture, Ecosystems & Environment, 100 (1), 63–74. doi: 10.1016/s0167-8809(03)00172-5
  11. Leip, A., Weiss, F., Lesschen, J. P., Westhoek, H. (2013). The nitrogen footprint of food products in the European Union. The Journal of Agricultural Science, 152 (S1), 20–33. doi: 10.1017/s0021859613000786
  12. Zasypka, L. H., Vorokhta, Yu. M., Stepanova, L. V., Babienko, V. V. (2008). Problema zabrudnennia ovochevoi produktsiy nitratamy. Problemy kharchuvannia, 3-4, 32–34.
  13. Valerko, R. A. (2013). Otsinka rivnia zabrudnennia roslynnytskoi produktsiy, vyroshchenoi v umovakh m. Zhytomyr. Visnyk derzhavnoho ahroekolohivchnoho universytetu, 1, 356–366.
  14. Tamme, T., Reinik, M., Roasto, M., Juhkam, K., Tenno, T., Kiis, A. (2006). Nitrates and nitrites in vegetables and vegetable-based products and their intakes by the Estonian population. Food Additives and Contaminants, 23 (4), 355–361. doi: 10.1080/02652030500482363
  15. Chung, S. Y., Kim, J. S., Kim, M., Hong, M. K., Lee, J. O., Kim, C. M., Song, I. S. (2003). Survey of nitrate and nitrite contents of vegetables grown in Korea. Food Additives and Contaminants, 20 (7), 621–628. doi: 10.1080/0265203031000124146
  16. Pardo-Marín, O., Yusà-Pelechà, V., Villalba-Martín, P., Perez-Dasí, J. A. (2010). Monitoring programme on nitrates in vegetables and vegetable-based baby foods marketed in the Region of Valencia, Spain: levels and estimated daily intake. Food Additives & Contaminants: Part A, 27 (4), 478–486. doi: 10.1080/19440040903439804
  17. Min, J., Zhang, H., Shi, W. (2012). Optimizing nitrogen input to reduce nitrate leaching loss in greenhouse vegetable production. Agricultural Water Management, 111, 53–59. doi: 10.1016/j.agwat.2012.05.003
  18. Inoue-Choi, M., Virk-Baker, M. K., Aschebrook-Kilfoy, B., Cross, A. J., Subar, A. F., Thompson, F. E. et. al. (2015). Development and calibration of a dietary nitrate and nitrite database in the NIH–AARP Diet and Health Study. Public Health Nutrition, 19 (11), 1934–1943. doi: 10.1017/s1368980015003407
  19. Bahadoran, Z., Mirmiran, P., Jeddi, S., Azizi, F., Ghasemi, A., Hadaegh, F. (2016). Nitrate and nitrite content of vegetables, fruits, grains, legumes, dairy products, meats and processed meats. Journal of Food Composition and Analysis, 51, 93–105. doi: 10.1016/j.jfca.2016.06.006
  20. Habermeyer, M., Roth, A., Guth, S., Diel, P., Engel, K.-H., Epe, B. et. al. (2014). Nitrate and nitrite in the diet: How to assess their benefit and risk for human health. Molecular Nutrition & Food Research, 59 (1), 106–128. doi: 10.1002/mnfr.201400286
  21. Kukhtyn, M., Horiuk, Y., Yaroshenko, T., Laiter-Moskaliuk, S., Levytska, V., Reshetnyk, A. (2018). Monitoring the content of nitrates in vegetables and the influence of the pickling technology on the denitrification process. EUREKA: Life Sciences, 1, 3–10. doi: 10.21303/2504-5695.2018.00528
  22. Musienko, M. T., Kryzhanivskyi, Ya. Y., Kukhtyn, M. D., Danylenko, I. P. (2008). Umist nitrativ u molotsi ta methemohlobinu v krovi koriv yak pokaznyk pozhyvnoi tsinnosti zymovykh ratsioniv. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohiy imeni S. Z. Gzhytskoho, 10 (3 (38)), 162–165.

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Published

2018-01-11

How to Cite

Kukhtyn, M., Horiuk, Y., Yaroshenko, T., Laiter-Moskaliuk, S., Levytska, V., & Reshetnyk, A. (2018). Effect of lactic acid microorganisms on the content of nitrates in tomato in the process of pickling. Eastern-European Journal of Enterprise Technologies, 1(11 (91), 69–75. https://doi.org/10.15587/1729-4061.2018.120548

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Section

Technology and Equipment of Food Production