Identification of Cronobacter spp (enterobacter sakazakii) from raw milk and environmental samples of dairy farms

Authors

DOI:

https://doi.org/10.15587/1729-4061.2017.114637

Keywords:

Cronobacter spp (formerly Enterobacter sakazakii), bulk tank milk, dairy farms, PCR, 16S rRNA

Abstract

The aim of this study was to identify the presence of Cronobacter spp (formerly Enterobacter sakazakii) in bulk tank milk and facilities of dairy farms in Sumy Region (Ukraine). A total of 418 samples of bulk tank milk, udder secretion of cows with a subclinical form of mastitis, and environmental samples were collected from 5 local dairy farms of Sumy region (Ukraine). All samples were analyzed for the presence of Cronobacter spp using the United States Food and Drug Administration (FDA) method. Twenty isolates were selected that had typical morphological, cultural and biochemical properties of Cronobacter spp and then they were subjected to a molecular analysis by polymerase chain reaction (PCR) amplification of 16S rRNA. The results indicate that the dairy farm environments, including the cows and the milk are a potential source of Cronobacter in raw milk. This is the first report of Cronobacter spp prevalence in bulk tank milk and environmental samples in the dairy farms of Sumy Region, Ukraine, indicating potential health risk associated with cow’s milk. These data will be valuable for microbiological risk assessment and help authorities to develop strategies to mitigate health risk for humans

Author Biographies

Oleksandra Berhilevych, Sumy State University Rymskoho-Korsakova str., 2, Sumy, Ukraine, 40007

Doctor of Veterinary Sciences, Professor

Department ofPublic Health

Victoria Kasianchuk, Sumy State University Rymskoho-Korsakova str., 2, Sumy, Ukraine, 40007

Doctor of Veterinary Sciences, Professor

Department ofPublic Health

References

  1. Iversen, C., Mullane, N., McCardell, B., Tall, B. D., Lehner, A., Fanning, S. et. al. (2008). Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. International Journal of Systematic and Evolutionary Microbiology, 58 (6), 1442–1447. doi: 10.1099/ijs.0.65577-0
  2. Joseph, S., Cetinkaya, E., Drahovska, H., Levican, A., Figueras, M. J., Forsythe, S. J. (2011). Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. International Journal of Systematic and Evolutionary Microbiology, 62 (Pt 6), 1277–1283. doi: 10.1099/ijs.0.032292-0
  3. Mullane, N. R., Iversen, C., Healy, B., Waish, C., Whyte, P., Wall, P. G., Quinn, T., Fanning, S. (2007). Enterobacter sakazakii: an emerging bacterial pathogen with implications for infant health. Minerva Pediatrica, 59 (2), 137–148.
  4. Yan, Q. Q., Condell, O., Power, K., Butler, F., Tall, B. D., Fanning, S. (2012). Cronobacter species (formerly known as Enterobacter sakazakii) in powdered infant formula: a review of our current understanding of the biology of this bacterium. Journal of Applied Microbiology, 113 (1), 1–15. doi: 10.1111/j.1365-2672.2012.05281.x
  5. Hunter, C. J., Bean, J. F. (2013). Cronobacter: an emerging opportunistic pathogen associated with neonatal meningitis, sepsis and necrotizing enterocolitis. Journal of Perinatology, 33 (8), 581–585. doi: 10.1038/jp.2013.26
  6. Enterobacter sakazakii and other microorganisms in powdered infant formula. Available at: http://www.who.int/foodsafety/publications/micro/es.pdf
  7. Shaker, R., Osaili, T., Al-Omary, W., Jaradat, Z., Al-Zuby, M. (2007). Isolation of Enterobacter sakazakii and other Enterobacter sp. from food and food production environments. Food Control, 18 (10), 1241–1245. doi: 10.1016/j.foodcont.2006.07.020
  8. Friedemann, M. (2007). Enterobacter sakazakii in food and beverages (other than infant formula and milk powder). International Journal of Food Microbiology, 116 (1), 1–10. doi: 10.1016/j.ijfoodmicro.2006.12.018
  9. Molloy, C., Cagney, C., O’Brien, S., Iversen, C., Fanning, S., Duffy, G. (2009). Surveillance and characterisation by Pulsed-Field Gel Electrophoresis of Cronobacter spp. in farming and domestic environments, food production animals and retail foods. International Journal of Food Microbiology, 136 (2), 198–203. doi: 10.1016/j.ijfoodmicro.2009.07.007
  10. Baumgartner, A., Grand, M., Liniger, M., Iversen, C. (2009). Detection and frequency of Cronobacter spp. (Enterobacter sakazakii) in different categories of ready-to-eat foods other than infant formula. International Journal of Food Microbiology, 136 (2), 189–192. doi: 10.1016/j.ijfoodmicro.2009.04.009
  11. Healy, B., Cooney, S., O’Brien, S., Iversen, C., Whyte, P., Nally, J. et. al. (2010). Cronobacter(Enterobacter sakazakii): An Opportunistic Foodborne Pathogen. Foodborne Pathogens and Disease, 7 (4), 339–350. doi: 10.1089/fpd.2009.0379
  12. Norrakiah, A. S., Odeyemi, O. A. (2015). Occurrence and prevalence of Cronobacter spp. in plant and animal derived food sources: a systematic review and meta-analysis. SpringerPlus, 4 (1), 545–555. doi: 10.1186/s40064-015-1324-9
  13. Molloy, C., Cagney, C., Fanning, S. (2008). Examination of cattle faeces, dairy farm and food environs for the presence of E. sakazakii. 21st International Committee for Food Microbiology and Hygiene Symposium, Evolving Microbial Food Quality and Safety. Aberdeen, 504.
  14. Molloy, C., Cagney, C., O’Brien, S., Iversen, C., Fanning, S., Duffy, G. (2009). Surveillance and characterisation by Pulsed-Field Gel Electrophoresis of Cronobacter spp. in farming and domestic environments, food production animals and retail foods. International Journal of Food Microbiology, 136 (2), 198–203. doi: 10.1016/j.ijfoodmicro.2009.07.007
  15. Bergilevich, O., Kasianchuk, V., Marchenko, A. (2013). The level of contamination raw cow's milk by microorganisms of the family Enterobacteriaceae including bacteria Enterobacter sakazakii at farms of Ukraine. Food Hygiene and Technology. 43st Lenfeld´s and Hökl´s Days, 100–103.
  16. Muytjens, H. L., Kollée, L. A. A. (1990). Enterobacter sakazakii meningitis in neonates. The Pediatric Infectious Disease Journal, 9 (5), 372. doi: 10.1097/00006454-199005000-00016
  17. Norrakiah, A. S. (2009). Cronobacter (Enterobacter) sakazakii. Enterobacteriaceae and aerobic plate count in raw and pasteurized milk. 1st International Conference on Cronobacter.
  18. Salman, A. M. A., Hamad, I. M. (2011). Enumeration and identification of Coliform bacteria from raw milk in Khartoum State, Sudan. Journal of Cell and Animal Biology, 5 (7), 121–128.
  19. Baumgartner, A., Niederhauser, I. (2010). Occurrence of Cronobacter spp. in raw milk. Journal Für Verbraucherschutz Und Lebensmittelsicherheit, 5 (2), 253–253. doi: 10.1007/s00003-010-0589-8
  20. Kandhai, M. C., Heuvelink, A. E., Reij, M. W., Beumer, R. R., Dijk, R., van Tilburg, J. J. H. C. et. al. (2010). A study into the occurrence of Cronobacter spp. in The Netherlands between 2001 and 2005. Food Control, 21 (8), 1127–1136. doi: 10.1016/j.foodcont.2010.01.007
  21. Lehner, A., Fricker-Feer, C., Gschwend, K., Stephan, R. (2010). Identification of Enterobacteriaceae and Cronobacter spp. in raw milk, milk concentrate and milk powder: prevalence and genotyping. Archiv für Lebensmittelhygiene, 61, 22–26.
  22. Buma, R., Sanada, H., Maeda, T., Kamei, M., Kourai, H. (1999). Isolation and characterization of pathogenic bacteria, including Escherichia coli O157 : H7,from flies collected at a dairy farm field. Medical Entomology and Zoology, 50 (4), 313–321. doi: 10.7601/mez.50.313
  23. Bergilevich, O., Kasianchuk, V., Deriabin, O. (2015). Identification of Cronobacter spp. (Enterobacter sakazakii) using PCR. Food Hygiene and Technology 45th Lenfeld's and Hökl's Days. Brno, 5–8. Available at: https://cit.vfu.cz/konference/lh2015/4download/sbornik.pdf
  24. Salmon, S. A., Watts, J. L., Aarestrup, F. M., Pankey, J. W., Yancey, R. J. (1998). Minimum Inhibitory Concentrations for Selected Antimicrobial Agents Against Organisms Isolated from the Mammary Glands of Dairy Heifers in New Zealand and Denmark. Journal of Dairy Science, 81 (2), 570–578. doi: 10.3168/jds.s0022-0302(98)75610-3
  25. Lehner, A., Nitzsche, S., Breeuwer, P., Diep, B., Thelen, K., Stephan, R. (2006). Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection. BMC Microbiology, 6 (1). doi: 10.1186/1471-2180-6-15
  26. Vineeth, B., Priyanka, B., Ramesh, B. S., Makari Hanumanthappa, K., Vinay Suvarna, M. N. (2014). Isolation and partial characterization of Cronobacter sakazakii by 16S rRNA sequence analysis isolated from milk of dairy cows with mastitis in Chikmagalur, Karnataka, India. International Journal of Scientific and Engineering Research, 5 (4), 671–680.
  27. Seo, K. H., Brackett, R. E. (2005). Rapid, Specific Detection of Enterobacter sakazakii in Infant Formula Using a Real-Time PCR Assay. Journal of Food Protection, 68 (1), 59–63. doi: 10.4315/0362-028x-68.1.59
  28. Isolation and enumeration of Enterobacter sakazakii from dehydrated powdered infant formula (2002). FDA. Available at: https://www.fda.gov/
  29. Liu, Y., Gao, Q., Zhang, X., Hou, Y., Yang, J., Huang, X. (2006). PCR and oligonucleotide array for detection of Enterobacter sakazakii in infant formula. Molecular and Cellular Probes, 20 (1), 11–17. doi: 10.1016/j.mcp.2005.08.004
  30. Friedemann, M. (2009). Epidemiology of invasive neonatal Cronobacter (Enterobacter sakazakii) infections. European Journal of Clinical Microbiology & Infectious Diseases, 28 (11), 1297–1304. doi: 10.1007/s10096-009-0779-4

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Published

2017-11-10

How to Cite

Berhilevych, O., & Kasianchuk, V. (2017). Identification of Cronobacter spp (enterobacter sakazakii) from raw milk and environmental samples of dairy farms. Eastern-European Journal of Enterprise Technologies, 6(11 (90), 4–10. https://doi.org/10.15587/1729-4061.2017.114637

Issue

Vol. 6 No. 11 (90) (2017): Technology and Equipment of Food Production

Section

Technology and Equipment of Food Production

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Copyright (c) 2017 Oleksandra Berhilevych, Victoria Kasianchuk

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