Development of the biotechnology for obtaining a dietary supplement from the selenium-containing probiotic cultures Lactobacillus acidophilus 412/307 and Bifidobacterium bifidum 1
Keywords:probiotics, dietary supplements, sodium selenite, bifidobacteria, lactobacilli, selenoproteins, optical density
The relevance of creating a new dietary supplement based on the selenium-containing cultures of lacto- and bifidobacteria was proven. We have chosen the optimal source of selenium – sodium selenite (Na2SeO3), adding which to the cultivation medium of microorganisms ensures maximum accumulation of organic forms of selenium in the examined microorganisms.
We have established the effect of concentrations of sodium selenite on an increase in the biomass of lacto- and bifidobacteria. Concentrations of Na2SeO3 exceeding 8 µg/cm3 cause the inhibition of growth in the lactobacilli biomass when compared with control. The growth of biomass of bifidobacteria is inhibited under the influence of the concentration of Na2SeO3 above 5 µg/cm3. Applying the indicators of optical density, we determined values for a specific growth rate and the duration of generation of biomass of the examined microorganisms. The dynamics of selenium accumulation by the cultures Lactobacillus acidophilus 412/307 and Bifidobacterium bifidum I was studied. A direct dependence was established between the quantitative content of inorganic selenium in the environment of cultivation and the content of organic selenium in bacterial cells. Adding the concentration of sodium selenite equal to 0.5 µg/cm3 provides for obtaining 105 µg of organic selenium per one gram of dry biomass of lactobacilli; this indicator for bifidobacteria is 97.5 µg/g. At a concentration of Na2SeO3 equal to 20 µg/cm3, 4,698 µg of organic selenium is biotransformed in the biomass of lactobacilli and 3,149 µg –in the biomass of bifidobacteria.Based on the data derived, we have developed a technological scheme for obtaining the dietary supplement with the quantitative content of organic selenium at 202.5±1 µg/g. The content of lactobacilli was 1.0×109 CFU/cm3; that of bifidobacteria – 1.2×108 CFU/cm3
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Copyright (c) 2018 Natalia Zykova, Leonid Kaprellyanz, Arsen Petrosyants, Alexander Zykov
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