Determining the influence of raw milk β-casein polymorphism on the efficiency of making soft cheese

Authors

DOI:

https://doi.org/10.15587/1729-4061.2024.311236

Keywords:

soft cheese, cheese yield, quality, acid-rennet technique, thermo-acid technique, β-casein, A2 milk

Abstract

One of the ways to increase the profitability of the cheese industry is the genetic selection of dairy cows to obtain milk with excellent cheese-capacity characteristics. The object of this study is the technology of fresh soft cheeses made by the acid-rennet and thermo-acid method from the milk of cows with different β-casein genotypes (A1A1, A1A2, A2A2). The subjects of research are the physicochemical indicators of raw milk from cows with different genotypes for β-casein (A1A1, A1A2, A2A2); as well as the yield of soft cheeses. The study has established that the physicochemical parameters of milk from cows with different β-casein genotypes are typical for fresh cow's milk. The study showed that with the acid-rennet method, the composition of cheeses from A1A1 milk was 51.60 %, 21.63 %, and 23.62 % of moisture, protein, and fat, respectively. A1A2 milk cheeses contained 50.70 % moisture, 20.96 % protein, and 25.12 % fat. A2A2 milk cheeses consisted of 52.50 % moisture, 20.70 % protein, and 23.71 % fat. With the thermo-acid method, cheeses from A1A1 milk were characterized by the moisture content of 55.13 %, proteins – 23.31 %, and fat – 20.21 %. A1A2 milk cheeses contained 58.13 %, 22.62 %, and 17.98 % of moisture, protein, and fat, respectively. A2A2 milk cheeses consisted of 54.03 % moisture, 22.33 % protein, and 22.25 % fat. The calculation of the production efficiency of soft cheeses from the milk of cows with different genotypes according to β-casein with the acid-rennet method of production is 119.3 % on average, which is more compared to milk A1A2 (by 4 %) and A2A2 (by 7 %). With the thermo-acidic method, the efficiency of cheese production from A2A2 milk is 107.5 %, which is higher compared to A1A2 milk (by 9 %) and A1A1 (by 5 %). The conclusions show that changes in the β-casein genotype in raw milk can affect the yield and quality of cheese, and therefore, the profitability of production

Author Biographies

Volodymyr Ladyka, Sumy National Agrarian University

Doctor of Agricultural Sciences, Professor, Academician of NAAS

Department of Technology of Production and Processing of Animal Products and Cinology

Nataliia Bolhova, Sumy National Agrarian University

PhD, Associate Professor

Department of Technology and Food Safety

Tetiana Synenko, Sumy National Agrarian University

Doctor of Philosophy (PhD)

Department of Technology and Food Safety

Yuriy Skliarenko, Institute of Agriculture of the Northeast of the National Academy of Sciences

Doctor of Agricultural Sciences

Laboratory of Animal Husbandry and Fodder Production

Viktoriia Vechorka, Sumy National Agrarian University

Doctor of Agricultural Sciences, Professor

Department of Animal Genetics, Breeding and Biotechnology

References

  1. OECD-FAO Agricultural Outlook 2023-2032. OECD-FAO Agricultural Outlook. https://doi.org/10.1787/08801ab7-en
  2. Sohail, Z., Khan, N., Moazzam, M., Mujahid, S., Tariq Sindhu, A., Khan, H. et al. (2024). Perspective Chapter: Beyond Delicious – The Hidden Functional Benefits of Cheese. Recent Trends on Cheese as Functional Food with Great Nutritive and Health Benefits. https://doi.org/10.5772/intechopen.113047
  3. Ladyka, V., Pavlenko, Y., Sklyarenko, Y. (2021). β-casein gene polymorphism use in terms of brown dairy cattle preservation. Archivos de Zootecnia, 70 (269), 87–94. https://doi.org/10.21071/az.v70i269.5422
  4. Samilyk, M., Tsyrulyk, R., Bolgova, N., Vechorka, V., Ryzhkova, T., Severin, R. et al. (2022). Devising a technique for improving the biological value of A2 milk by adding carrot powder. Eastern-European Journal of Enterprise Technologies, 6 (11 (120)), 44–50. https://doi.org/10.15587/1729-4061.2022.266924
  5. Banerjee, S. (2018). A2 milk: the unknown story about a milk protein. Acta Scientific Nutritional Health, 2 (3), 28–31. Available at: https://actascientific.com/ASNH/pdf/ASNH-02-0057.pdf
  6. Cipolat-Gotet, C., Cecchinato, A., Malacarne, M., Bittante, G., Summer, A. (2018). Variations in milk protein fractions affect the efficiency of the cheese-making process. Journal of Dairy Science, 101 (10), 8788–8804. https://doi.org/10.3168/jds.2018-14503
  7. Daniloski, D., McCarthy, N. A., Huppertz, T., Vasiljevic, T. (2022). What is the impact of amino acid mutations in the primary structure of caseins on the composition and functionality of milk and dairy products? Current Research in Food Science, 5, 1701–1712. https://doi.org/10.1016/j.crfs.2022.09.026
  8. Sebastiani, C., Arcangeli, C., Torricelli, M., Ciullo, M., D’avino, N., Cinti, G. et al. (2022). Marker-assisted selection of dairy cows for β-casein gene A2 variant. Italian Journal of Food Science, 34 (2), 21–27. https://doi.org/10.15586/ijfs.v34i2.2178
  9. Daniloski, D., McCarthy, N. A., Vasiljevic, T. (2021). Bovine β-Casomorphins: Friends or Foes? A comprehensive assessment of evidence from in vitro and ex vivo studies. Trends in Food Science & Technology, 116, 681–700. https://doi.org/10.1016/j.tifs.2021.08.003
  10. Hohmann, L. G., Weimann, C., Scheper, C., Erhardt, G., König, S. (2021). Genetic diversity and population structure in divergent German cattle selection lines on the basis of milk protein polymorphisms. Archives Animal Breeding, 64 (1), 91–102. https://doi.org/10.5194/aab-64-91-2021
  11. Sun, Y., Ding, Y., Liu, B., Guo, J., Su, Y., Yang, X. et al. (2024). Recent advances in the bovine β-casein gene mutants on functional characteristics and nutritional health of dairy products: Status, challenges, and prospects. Food Chemistry, 443, 138510. https://doi.org/10.1016/j.foodchem.2024.138510
  12. Ladyka, V., Bolgova, N., Synenko, T., Skliarenko, Y., Vechorka, V. (2023). Determining the influence of raw milk protein composition on the yield of cheese and its nutrient content. Eastern-European Journal of Enterprise Technologies, 6 (11 (126)), 33–41. https://doi.org/10.15587/1729-4061.2023.292063
  13. Juan, B., Trujillo, A.-J. (2022). Acid and Rennet Coagulation Properties of A2 Milk. Foods, 11 (22), 3648. https://doi.org/10.3390/foods11223648
  14. Nguyen, H. T. H., Schwendel, H., Harland, D., Day, L. (2018). Differences in the yoghurt gel microstructure and physicochemical properties of bovine milk containing A1A1 and A2A2 β-casein phenotypes. Food Research International, 112, 217–224. https://doi.org/10.1016/j.foodres.2018.06.043
  15. Oliveira Mendes, M., Ferreira de Morais, M., Ferreira Rodrigues, J. (2019). A2A2 milk: Brazilian consumers’ opinions and effect on sensory characteristics of Petit Suisse and Minas cheeses. LWT, 108, 207–213. https://doi.org/10.1016/j.lwt.2019.03.064
  16. Bisutti, V., Pegolo, S., Giannuzzi, D., Mota, L. F. M., Vanzin, A., Toscano, A. et al. (2022). The β-casein (CSN2) A2 allelic variant alters milk protein profile and slightly worsens coagulation properties in Holstein cows. Journal of Dairy Science, 105 (5), 3794–3809. https://doi.org/10.3168/jds.2021-21537
  17. Gustavsson, F., Buitenhuis, A. J., Glantz, M., Stålhammar, H., Lindmark-Månsson, H., Poulsen, N. A. et al. (2014). Impact of genetic variants of milk proteins on chymosin-induced gelation properties of milk from individual cows of Swedish Red dairy cattle. International Dairy Journal, 39 (1), 102–107. https://doi.org/10.1016/j.idairyj.2014.05.007
  18. de Vitte, K., Kerziene, S., Klementavičiūtė, J., de Vitte, M., Dilbiene, V., Stankevičius, R. (2022). Relationship between β-casein genotypes (A1A1, A1A2, and A2A2) and coagulation properties of milk and the fatty acid composition and sensory characteristics of dairy products (soft cheese, sour cream, and butter). Acta Agriculturae Scandinavica, Section A – Animal Science, 71 (1-4), 21–32. https://doi.org/10.1080/09064702.2022.2141308
  19. Stocco, G., Cipolat-Gotet, C., Gasparotto, V., Cecchinato, A., Bittante, G. (2018). Breed of cow and herd productivity affect milk nutrient recovery in curd, and cheese yield, efficiency and daily production. Animal, 12 (2), 434–444. https://doi.org/10.1017/s1751731117001471
  20. Vigolo, V., Franzoi, M., Penasa, M., De Marchi, M. (2022). β-Casein variants differently affect bulk milk mineral content, protein composition, and technological traits. International Dairy Journal, 124, 105221. https://doi.org/10.1016/j.idairyj.2021.105221
  21. Niero, G., Visentin, G., Ton, S., De Marchi, M., Penasa, M., Cassandro, M. (2016). Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed. Italian Journal of Animal Science, 15 (4), 576–583. https://doi.org/10.1080/1828051x.2016.1250128
  22. Ladyka, V. I., Pavlenko, Y. M., Sklyarenko, Y. I., Ladyka, L. M., Levchenko, I. V. (2022). Influence of beta-casein genotype on milk quality indicators in brown cattle. Bulletin of Sumy National Agrarian University. The Series: Livestock, 4 (47), 7–12. https://doi.org/10.32845/bsnau.lvst.2021.4.2
  23. Guinee, T. P., Mulholland, E. O., Kelly, J., Callaghan, D. J. O. (2007). Effect of Protein-to-Fat Ratio of Milk on the Composition, Manufacturing Efficiency, and Yield of Cheddar Cheese. Journal of Dairy Science, 90 (1), 110–123. https://doi.org/10.3168/jds.s0022-0302(07)72613-9
  24. Gislon, G., Bava, L., Bisutti, V., Tamburini, A., Brasca, M. (2023). Bovine beta casein polymorphism and environmental sustainability of cheese production: The case of Grana Padano PDO and mozzarella cheese. Sustainable Production and Consumption, 35, 85–94. https://doi.org/10.1016/j.spc.2022.10.017
  25. Jensen, H. B., Holland, J. W., Poulsen, N. A., Larsen, L. B. (2012). Milk protein genetic variants and isoforms identified in bovine milk representing extremes in coagulation properties. Journal of Dairy Science, 95 (6), 2891–2903. https://doi.org/10.3168/jds.2012-5346
  26. Sturaro, A., De Marchi, M., Zorzi, E., Cassandro, M. (2015). Effect of microparticulated whey protein concentration and protein-to-fat ratio on Caciotta cheese yield and composition. International Dairy Journal, 48, 46–52. https://doi.org/10.1016/j.idairyj.2015.02.003
  27. Marko, R., Uros, G., Branislav, V., Milan, M., Danijela, K., Vlado, T., Zoran, S. (2020). Beta-Casein Gene Polymorphism in Serbian Holstein-Friesian Cows and Its Relationship with Milk Production Traits. Acta Veterinaria, 70 (4), 497–510. https://doi.org/10.2478/acve-2020-0037
Determining the influence of raw milk β-casein polymorphism on the efficiency of making soft cheese

Downloads

Published

2024-10-30

How to Cite

Ladyka, V., Bolhova, N., Synenko, T., Skliarenko, Y., & Vechorka, V. (2024). Determining the influence of raw milk β-casein polymorphism on the efficiency of making soft cheese. Eastern-European Journal of Enterprise Technologies, 5(11 (131), 33–42. https://doi.org/10.15587/1729-4061.2024.311236

Issue

Section

Technology and Equipment of Food Production