Determining the effect of high power pulse ultraviolet (UV) radiation on Bacillus anthracis

Authors

DOI:

https://doi.org/10.15587/1729-4061.2025.322730

Keywords:

bactericidal effect, disinfection, environmental sanitation, anthrax, spores, UV radiation at different pulses

Abstract

The object of this study is the high-power UV radiation at different pulses and its effect on Bacillus anthracis spores. The destruction of pathogenic microorganisms at environmental objects is the key to sustainable epizootological well-being. The results of the study show that the effect of powerful pulses on the destruction of pathogenic microorganisms is achieved during a short series and even single exposures. In this case, a pulsed UV sterilizer based on a magnetoplasma compressor with a pulsed ultraviolet flux power of 3.6 MW with a wavelength of 185‒320 nm of ultraviolet radiation was used. The prospects for pulsed sterilization technology have been shown. As a result of laboratory tests, it was established that the elimination degree of spores of the test microorganism Bacillus anthracis CTI, applied to wooden plates, is lg 2.5; applied to plastic plates – lg 3.2, while the degree of death of spores applied to metal plates is lg 4.0. Based on the results, wood and plastic require more attention when choosing disinfection methods due to their lower efficiency in this context. The high resistance of spore microorganisms requires the use of high dose loads to neutralize the anthrax pathogen.

The proposed disinfection methodology is relevant and can be used by veterinary medicine laboratories, livestock farms of various forms of ownership, the scientific community, and industrial enterprises manufacturing technological equipment. Further research should be aimed at devising and improving methods for combating pathogenic microorganisms and ensuring sustainable epizootic well-being

Author Biographies

Volodymyr Chumakov, Astronomical Observatory of Brera

Doctor of Technical Sciences, Professor

Scientific Department

National Institute for Astrophysics (INAF)

Nataliia Pinchuk, BIOTESTLAB

PhD, Senior Researcher

Oksana Kharchenko, Astronomical Observatory of Brera

PhD, Senior Researcher

National Institute for Astrophysics (INAF)

Vasyl Muraveynik, Limited Liability Company "Triix"

Director

Mykhailo Ostrizhnyi, Limited Liability Company "Triix"

Engineer

Vitaliy Tsymbalyuk, National Academy of Medical Sciences of Ukraine

Doctor of Medical Sciences, Academician of the National Academy of Medical Sciences of Ukraine

President

Nadiia Polka, Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine

Doctor of Medical Sciences, Corresponding Member of the National Academy of Sciences of Ukraine, Deputy Director for Scientific Work

Vitaliy Bolotin, State Scientific and Control Institute of Biotechnology and Strains of Microorganisms

PhD, Senior Researcher, Deputy Director for Scientific Work

Oleksandr Kornieikov, State Scientific and Control Institute of Biotechnology and Strains of Microorganisms

PhD, Head of Department

Department of Biotechnology and Quality Control of Viral Preparations

Anatoliy Paliy, National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"

Doctor of Veterinary Sciences, Professor

Director

References

  1. Paliy, A., Aliiev, E., Paliy, A., Ishchenko, K., Shkromada, O., Musiienko, Y. et al. (2021). Development of a device for cleansing cow udder teats and testing it under industrial conditions. Eastern-European Journal of Enterprise Technologies, 1 (1 (109)), 43–53. https://doi.org/10.15587/1729-4061.2021.224927
  2. Hernandez-Patlan, D., Tellez-Isaias, G., Hernandez-Velasco, X., Solis-Cruz, B. (2023). Editorial: Technological strategies to improve animal health and production. Frontiers in Veterinary Science, 10. https://doi.org/10.3389/fvets.2023.1206170
  3. Mata, F. (2021). A Framework for Using Epidemiology in Animal Welfare Science. Journal of Applied Animal Welfare Science, 26 (3), 361–373. https://doi.org/10.1080/10888705.2021.1981902
  4. Rozman, U., Pušnik, M., Kmetec, S., Duh, D., Šostar Turk, S. (2021). Reduced Susceptibility and Increased Resistance of Bacteria against Disinfectants: A Systematic Review. Microorganisms, 9 (12), 2550. https://doi.org/10.3390/microorganisms9122550
  5. Paliy, A. P. (2018). Differential Sensitivity of Mycobacterium to Chlorine Disinfectants. Mikrobiolohichnyi Zhurnal, 80 (2), 104–116. https://doi.org/10.15407/microbiolj80.02.104
  6. Korniienko, L. Y., Ukhovskyi, V. V., Moroz, O. A., Chechet, O. M., Haidei, O. S., Tsarenko, T. M. et al. (2022). Epizootological and epidemiological situation of anthrax in Ukraine in the context of mandatory specific prevention in susceptible animals. Regulatory Mechanisms in Biosystems, 13 (4), 346–353. https://doi.org/10.15421/022245
  7. Dong, F., Chen, J., Li, C., Ma, X., Jiang, J., Lin, Q. et al. (2019). Evidence-based analysis on the toxicity of disinfection byproducts in vivo and in vitro for disinfection selection. Water Research, 165, 114976. https://doi.org/10.1016/j.watres.2019.114976
  8. Rodionova, K., Khimych, M., Paliy, A. (2021). Veterinary and sanitary assessment and disinfection of refrigerator chambers of meat processing enterprises. Potravinarstvo Slovak Journal of Food Sciences, 15, 616–626. https://doi.org/10.5219/1628
  9. Tong, C., Hu, H., Chen, G., Li, Z., Li, A., Zhang, J. (2021). Disinfectant resistance in bacteria: Mechanisms, spread, and resolution strategies. Environmental Research, 195, 110897. https://doi.org/10.1016/j.envres.2021.110897
  10. Sandri, A., Tessari, A., Giannetti, D., Cetti, A., Lleo, M. M., Boschi, F. (2023). UV-A Radiation: Safe Human Exposure and Antibacterial Activity. International Journal of Molecular Sciences, 24 (9), 8331. https://doi.org/10.3390/ijms24098331
  11. Sliney, D. H., Stuck, B. E. (2021). A Need to Revise Human Exposure Limits for Ultraviolet UV‐C Radiation. Photochemistry and Photobiology, 97 (3), 485–492. https://doi.org/10.1111/php.13402
  12. Guidelines on limits of exposure to ultraviolet radiation of wavelengths between 180 nm and 400 nm (incoherent optical radiation) (2004). Health Physics, 87 (2), 171–186. https://doi.org/10.1097/00004032-200408000-00006
  13. Bourouiba, L. (2020). Turbulent Gas Clouds and Respiratory Pathogen Emissions. JAMA, 323 (18), 1837–1838. https://doi.org/10.1001/jama.2020.4756
  14. Paliy, A. P., Sumakova, N. V., Mashkey, A. M., Petrov, R. V., Paliy, A. P., Ishchenko, K. V. (2018). Contamination of animal-keeping premises with eggs of parasitic worms. Biosystems Diversity, 26 (4), 327–333. https://doi.org/10.15421/011848
  15. Paliy, A., Sumakova, N., Petrov, R., Shkromada, O., Ulko, L., Palii, A. (2019). Contamination of urbanized territories with eggs of helmiths of animals. Biosystems Diversity, 27 (2), 118–124. https://doi.org/10.15421/011916
  16. Zhang, H. J., Han, Q. Y., Zhang, S. D. (2013). 254 nm Radiant Efficiency of High Output Low Pressure Mercury Discharge Lamps with Neon-Argon Buffer Gas. Applied Mechanics and Materials, 325-326, 409–412. https://doi.org/10.4028/www.scientific.net/amm.325-326.409
  17. Rowe, J. P., Lambe, A. T., Brune, W. H. (2020). Technical Note: Effect of varying the λ = 185 and 254 nm photon flux ratio on radical generation in oxidation flow reactors. Atmospheric Chemistry and Physics, 20 (21), 13417–13424. https://doi.org/10.5194/acp-20-13417-2020
  18. Terri, M., Mancianti, N., Trionfetti, F., Casciaro, B., de Turris, V., Raponi, G. et al. (2022). Exposure to b-LED Light While Exerting Antimicrobial Activity on Gram-Negative and -Positive Bacteria Promotes Transient EMT-like Changes and Growth Arrest in Keratinocytes. International Journal of Molecular Sciences, 23 (3), 1896. https://doi.org/10.3390/ijms23031896
  19. Gerchman, Y., Mamane, H., Friedman, N., Mandelboim, M. (2020). UV-LED disinfection of Coronavirus: Wavelength effect. Journal of Photochemistry and Photobiology B: Biology, 212, 112044. https://doi.org/10.1016/j.jphotobiol.2020.112044
  20. Kowalski, W. (2009). UV Effects on Materials. Ultraviolet Germicidal Irradiation Handbook, 361–381. https://doi.org/10.1007/978-3-642-01999-9_15
  21. van der Starre, C. M., Cremers-Pijpers, S. A. J., van Rossum, C., Bowles, E. C., Tostmann, A. (2022). The in situ efficacy of whole room disinfection devices: a literature review with practical recommendations for implementation. Antimicrobial Resistance & Infection Control, 11 (1). https://doi.org/10.1186/s13756-022-01183-y
  22. Knudson, G. B. (1986). Photoreactivation of ultraviolet-irradiated, plasmid-bearing, and plasmid-free strains of Bacillus anthracis. Applied and Environmental Microbiology, 52 (3), 444–449. https://doi.org/10.1128/aem.52.3.444-449.1986
  23. Rao, B. K., Kumar, P., Rao, S., Gurung, B. (2011). Bactericidal effect of ultraviolet C (UVC), direct and filtered through transparent plastic, on gram-positive cocci: an in vitro study. Ostomy/Wound Management, 57 (7), 46–52.
  24. Chumakov, V. I., Slichenko, N. I., Stolarhuk, A. V., Egorov, A. M., Lonin, Yu. F. (2004). Simulation of the thermal mechanism in semiconductors under action of pulsed electromagnetic field. Problems of Atomic Sience and Technology, 2, 203–205.
  25. Chumakov, V. I. (2015). Pat. No. 104719 UA. Pulse Sterilizer. No. u201508907; declareted: 15.09.2015; published: 10.02.2016, Bul. No. 3.
  26. Wood, J. P., Archer, J., Calfee, M. W., Serre, S., Mickelsen, L., Mikelonis, A. et al. (2020). Inactivation of Bacillus anthracis and Bacillus atrophaeus spores on different surfaces with ultraviolet light produced with a low-pressure mercury vapor lamp or light emitting diodes. Journal of Applied Microbiology, 131 (5), 2257–2269. https://doi.org/10.1111/jam.14791
  27. Oettler, M. J., Conraths, F. J., Roesler, U., Reiche, S., Homeier-Bachmann, T., Denzin, N. (2024). Efficiency of Virucidal Disinfectants on Wood Surfaces in Animal Husbandry. Microorganisms, 12 (5), 1019. https://doi.org/10.3390/microorganisms12051019
Determining the effect of high power pulse ultraviolet (UV) radiation on Bacillus anthracis

Downloads

Published

2025-02-28

How to Cite

Chumakov, V., Pinchuk, N., Kharchenko, O., Muraveynik, V., Ostrizhnyi, M., Tsymbalyuk, V., Polka, N., Bolotin, V., Kornieikov, O., & Paliy, A. (2025). Determining the effect of high power pulse ultraviolet (UV) radiation on Bacillus anthracis. Eastern-European Journal of Enterprise Technologies, 1(5 (133), 6–11. https://doi.org/10.15587/1729-4061.2025.322730

Issue

Vol. 1 No. 5 (133) (2025): Applied physics

Section

Applied physics

License

Copyright (c) 2025 Volodymyr Chumakov, Nataliіa Pinchuk, Oksana Kharchenko, Vasyl Muraveynik, Mykhailo Ostrizhnyi, Vitaliy Tsymbalyuk, Nadiia Polka, Vitaliy Bolotin, Oleksandr Kornieikov, Anatoliy Paliy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The consolidation and conditions for the transfer of copyright (identification of authorship) is carried out in the License Agreement. In particular, the authors reserve the right to the authorship of their manuscript and transfer the first publication of this work to the journal under the terms of the Creative Commons CC BY license. At the same time, they have the right to conclude on their own additional agreements concerning the non-exclusive distribution of the work in the form in which it was published by this journal, but provided that the link to the first publication of the article in this journal is preserved.

A license agreement is a document in which the author warrants that he/she owns all copyright for the work (manuscript, article, etc.).
The authors, signing the License Agreement with TECHNOLOGY CENTER PC, have all rights to the further use of their work, provided that they link to our edition in which the work was published.
According to the terms of the License Agreement, the Publisher TECHNOLOGY CENTER PC does not take away your copyrights and receives permission from the authors to use and dissemination of the publication through the world's scientific resources (own electronic resources, scientometric databases, repositories, libraries, etc.).
In the absence of a signed License Agreement or in the absence of this agreement of identifiers allowing to identify the identity of the author, the editors have no right to work with the manuscript.
It is important to remember that there is another type of agreement between authors and publishers – when copyright is transferred from the authors to the publisher. In this case, the authors lose ownership of their work and may not use it in any way.