Development of method of use of acid-resistant selective action enzyme preparations
DOI:
https://doi.org/10.15587/1729-4061.2015.46501Keywords:
mash, enzyme preparations, fermentation of wort, wort, water-soluble carbohydrates, insoluble starch, concentrationAbstract
A lot of studies were conducted nowadays in distilling industry on biocatalysis and fermentation of wort from starch-containing raw material using selective action enzyme complexes, but operational parameters for hydrolysis of starch-containing raw material biopolymers are not sufficiently investigated at low pH of the substrate. On the market of enzyme preparations in Ukraine there are acid-resistant enzymes that enable hydrolysis of grain mashes in low pH environment. This article concentrates on determination of efficiency of use of acid-resistant enzyme preparations (EP) when producing worts from starch-containing raw material. The results of studies on content in wort of water-soluble carbohydrates and insoluble starch depending on pH, temperature of termofermentive processing (TFP) and enzyme activity stabilizer Ca2+ ions are demonstrated. In terms of bioconversion efficiency of raw grain material components and assurance of microbiological purity of intermediate products of alcohol production, optimum pH is 3.8 - 4.0, mash processing time is 90 minutes.
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Copyright (c) 2015 Петро Леонідович Шиян, Тетяна Омелянівна Мудрак, Анатолій Михайлович Куц, Ярослав Андрійович Боярчук
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