Quantitative determination of vitamin C in model food systems
DOI:
https://doi.org/10.15587/1729-4061.2012.5617Keywords:
vitamin C determination method, chemical composition, food systemAbstract
The quantitative analysis of vitamin C is of a certain difficulty, and today there is no universal serial method free from defects for the analysis. The perfect method of analysis should provide the ability to determine simultaneously both the L-ascorbic acid and its various forms, and find distinct differences between these compounds.
As a rule, during the analysis of vitamin C, the unique property of L-ascorbic acid to participate in redox reactions is applied (e.g. Tilmans’ method). All other forms of vitamin C (oxidized form - L-dehydroascorbic acid and related form – ascorbic gene) remain outside the definition and results of analyses in most cases are understated.
Also, in our opinion, the interaction of vitamin C with other components of food systems is a possible source of errors.
It is known that starch absorbs relatively small amount of acids from solutions (up to 3%), so the results obtained cannot be explained only by adsorption of ascorbic acid on starch. Another possible explanation of ascorbic acid oxidation to dehydroascorbic acid is also wrong, because at the initial stage of the experiment it was showed that pretreatment of the sample with cyst deoxidizer does not change the results of determination.
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