Evaluation of quality indicators and composition of semi-smoked sausage from plant-based meat substitute enriched with blood of slaughtered animals
DOI:
https://doi.org/10.15587/1729-4061.2025.336087Keywords:
semi-smoked sausage, meat substitute, blood of slaughtered animals, physicochemical compositionAbstract
Object of the study is semi-smoked sausage from a plant-based meat substitute, enriched with blood of slaughtered animals in various proportions.
The problem to be solved is that the blood of farm animals, being a valuable source of protein, is thrown away by the tons as waste, and is aggravated by the emerging serious problems of environmental pollution. But in many countries, most of the blood proteins are used as binders, natural color enhancers, emulsifiers, fat substitutes and agents for salting meat.
The article presents the results of the study of organoleptic evaluation, mineral and physicochemical composition of semi-smoked sausage from plant-based meat substitute enriched with blood of slaughtered animals. Soybeans and chickpeas were selected as plant raw materials in a ratio of 70:30. According to the results of the organoleptic evaluation, it was found that the addition of blood of slaughtered animals into the recipe of investigational product in the amount of 2%, improves the taste and color of the finished product. In the course of the study it was proved that when 2% dry blood of slaughtered animals was added to a semi-smoked sausage from plant-based meat substitute, the amount of protein increased by 2.26% compared with the control sample. Also, the increased fat content in the investigational samples of semi-smoked sausage showed that the blood of slaughtered animals can be used as a fat substitute. Based on studies of the mineral composition of semi-smoked sausage from plant-based meat substitutes, it has been proven that adding 2–6% blood of slaughtered animal increases the content of mineral substances compared to the control sample. Studies have shown that adding 2%, 4%, 6% blood of slaughtered animals to a semi-smoked sausage from plant-based meat substitute increases the content of essential and non-essential amino acids compared to the control sample. It was found that in experimental samples with the addition of 2%, 4%, 6% blood of slaughtered animals, the content of saturated and polyunsaturated fatty acids increases compared with the control sample. Thereby enriching and increasing the biological and nutritional value of the finished product
References
- Zheleuova, Z. S., Uzakov, Y. M., Shingisov, A. U., Alibekov, R. S., Khamitova, B. M. (2020). Development of halal cooked smoked beef and turkey sausage using a combined plant extracts. Journal of Food Processing and Preservation, 45 (1). https://doi.org/10.1111/jfpp.15028
- Cha, S.-H., Shin, K.-O., Han, K.-S. (2020). A study on the physicochemical properties of sausage analogue made with mixed bean protein concentrate. Korean journal of Food science and Technology, 52 (6), 641–648. https://doi.org/10.9721/KJFST.2020.52.6.641
- Joshi, V., Kumar, S. (2015). Meat Analogues: Plant based alternatives to meat products- A review. International Journal of Food and Fermentation Technology, 5 (2), 107. https://doi.org/10.5958/2277-9396.2016.00001.5
- Zheleuova, Z., Shingisov, A., Berdenbetova, A., Balabekova, A., Imanbayev, A. (2025). Developing a sausage product which have increased nutritional value and improved amino acid properties. Eastern-European Journal of Enterprise Technologies, 2 (11 (134)), 96–103. https://doi.org/10.15587/1729-4061.2025.327439
- Padha, N., Salve, R. V., Gaurav, D. (2024). Legume-Based Meat Analog Products: A Comprehensive Analysis of Nutritional and Technological Aspects. Bioscene, 21 (02), 1117–1132. Available at: https://explorebioscene.com/assets/uploads/doc/ae7e6-1116-1132.10000173.pdf
- Kurek, M. A., Onopiuk, A., Pogorzelska-Nowicka, E., Szpicer, A., Zalewska, M., Półtorak, A. (2022). Novel Protein Sources for Applications in Meat-Alternative Products – Insight and Challenges. Foods, 11 (7), 957. https://doi.org/10.3390/foods11070957
- Mazumder, Md., Sujintonniti, N., Chaum, P., Ketnawa, S., Rawdkuen, S. (2023). Developments of Plant-Based Emulsion-Type Sausage by Using Grey Oyster Mushrooms and Chickpeas. Foods, 12 (8), 1564. https://doi.org/10.3390/foods12081564
- Boukid, F. (2021). Chickpea (Cicer arietinum L.) protein as a prospective plant‐based ingredient: a review. International Journal of Food Science & Technology, 56 (11), 5435–5444. https://doi.org/10.1111/ijfs.15046
- Appiah, J., Peggy, Y.-H. (2012). The Use of Blood and Derived Products as Food Additives. Food Additive. https://doi.org/10.5772/32374
- Bohrer, B. M. (2023). Blood and Blood Constituents for Meat Processing. Current Food Science and Technology Reports, 2 (1), 17–25. https://doi.org/10.1007/s43555-023-00015-3
- Rodriguez Furlán, L. T., Padilla, A. P., Campderrós, M. E. (2014). Development of reduced fat minced meats using inulin and bovine plasma proteins as fat replacers. Meat Science, 96 (2), 762–768. https://doi.org/10.1016/j.meatsci.2013.09.015
- Kumari, S., Pandey, A., Soni, A., Sarkar, S., Kumar, A. (2024). Effect of plasma protein hydrolysates on fatty acids, colour, texture and oxidation stability of mutton sausage during refrigeration storage. Indian Journal of Small Ruminants (The), 30 (1), 129–137. https://doi.org/10.5958/0973-9718.2024.00030.8
- Oro, C. E. D., Rigo, D., Gaio, I., Valduga, E., Paliga, M., Silva, M. F. et al. (2018). Formulation of chicken sausages with broiler blood proteins and dye. Journal of Food Science and Technology, 55 (11), 4694–4699. https://doi.org/10.1007/s13197-018-3403-8
- Goemaere, O., Glorieux, S., Govaert, M., Steen, L., Fraeye, I. (2021). Phosphate Elimination in Emulsified Meat Products: Impact of Protein-Based Ingredients on Quality Characteristics. Foods, 10 (4), 882. https://doi.org/10.3390/foods10040882
- Cofrades, S., Guerra, M. A., Carballo, J., Fernández‐Martín, F., Colmenero, F. J. (2000). Plasma Protein and Soy Fiber Content Effect on Bologna Sausage Properties as Influenced by Fat Level. Journal of Food Science, 65 (2), 281–287. https://doi.org/10.1111/j.1365-2621.2000.tb15994.x
- Furlán, L. T. R., Padilla, A. P., Campderrós, M. E. (2010). Functional and physical properties of bovine plasma proteins as a function of processing and pH, application in a food formulation. Advance Journal of Food Science and Technology, 2 (5), 256–267. Available at: https://maxwellsci.com/print/ajfst/v2-256-267.pdf
- Lennon, A. M., McDonald, K., Moon, S. S., Ward, P., Kenny, T. A. (2010). Performance of cold-set binding agents in re-formed beef steaks. Meat Science, 85 (4), 620–624. https://doi.org/10.1016/j.meatsci.2010.03.014
- Bah, C. S., Bekhit, A. E. A., Carne, A., McConnell, M. A. (2015). Composition and biological activities of slaughterhouse blood from red deer, sheep, pig and cattle. Journal of the Science of Food and Agriculture, 96 (1), 79–89. https://doi.org/10.1002/jsfa.7062
- Bah, C. S. F., Bekhit, A. E. A., Carne, A., McConnell, M. A. (2013). Slaughterhouse Blood: An Emerging Source of Bioactive Compounds. Comprehensive Reviews in Food Science and Food Safety, 12 (3), 314–331. https://doi.org/10.1111/1541-4337.12013
- ISO 12966-1:2014. Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (2014). Geneva: International Organization for Standardization, 17.
- AOAC Official Method 994.12: Amino Acids in Feeds — Ion-exchange chromatography method. Chap. 45 (2000). Gaithersburg, MD: AOAC International.
- Mathi, P. (2016). Development of beef sausages through bovine blood utilization as fat replacer to reduce slaughterhouse by-product losses. University of Nairobi, 100.
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