Devising of a system for analysing the dispersed composition of emulsions using computer vision methods
DOI:
https://doi.org/10.15587/1729-4061.2026.352589Keywords:
image segmentation, emulsion analysis, computer vision, droplet distribution, droplet diameterAbstract
The feasibility of applying computer vision sequences to automatically determine the composition of heterogeneous disperse systems, using emulsions as a case study, has been considered. This expands the analytical framework, reduces human factor impact on analysis accuracy and reliability, as well as improves processing speed.
During the study, zero-shot segmentation was performed on microscopy images using four different segmenters. The resulting segments were then fitted to circles using a bounding volume (BV) approach. Segmentation effectiveness was evaluated with the Intersection over Union (IoU) metric by comparing results to manually annotated masks provided by an operator.
The average IoU values for the applied segmentation models range from 0.64 to 0.68. Applying the BV technique improves agreement with reference masks; specifically, the average IoU fitted to circles reaches approximately 0.75.
The overall effectiveness of applying the proposed automatic system in the form of a segmentation and bounding volume sequence was determined by analyzing the emulsion droplet diameter distributions. Comparison of the distributions showed that the data obtained using the automatic system are consistent with the operator's data for fractions larger than 15 px. However, the automatic system underestimates the share of fine fractions, which leads to a systematic shift in the integral assessment.
Importantly, it was established that applying the BV method to each individual mask obtained from segmentation is approximately 40–60% faster than analyzing a single combined mask. This analysis of individual masks is also practically more useful in cases involving touching droplets
References
- Kirillov, A., Mintun, E., Ravi, N., Mao, H., Rolland, C., Gustafson, L. et al. (2023). Segment Anything. 2023 IEEE/CVF International Conference on Computer Vision (ICCV), 3992–4003. https://doi.org/10.1109/iccv51070.2023.00371
- Ma, J., He, Y., Li, F., Han, L., You, C., Wang, B. (2024). Segment anything in medical images. Nature Communications, 15 (1). https://doi.org/10.1038/s41467-024-44824-z
- Zhang, Y., Jiang, H., Ye, T., Juhas, M. (2021). Deep Learning for Imaging and Detection of Microorganisms. Trends in Microbiology, 29 (7), 569–572. https://doi.org/10.1016/j.tim.2021.01.006
- Nartova, A. V., Mashukov, M. Yu., Astakhov, R. R., Kudinov, V. Yu., Matveev, A. V., Okunev, A. G. (2022). Particle Recognition on Transmission Electron Microscopy Images Using Computer Vision and Deep Learning for Catalytic Applications. Catalysts, 12 (2), 135. https://doi.org/10.3390/catal12020135
- Salum, P., Güven, O., Aydemir, L. Y., Erbay, Z. (2022). Microscopy-Assisted Digital Image Analysis with Trainable Weka Segmentation (TWS) for Emulsion Droplet Size Determination. Coatings, 12 (3), 364. https://doi.org/10.3390/coatings12030364
- Richards, K. D., Comish, E., Evans, R. C. (2025). Computer vision for high-throughput analysis of pickering emulsions. Soft Matter, 21 (12), 2339–2348. https://doi.org/10.1039/d4sm01252f
- Saalbrink, J., Loo, T. Y. J., Mertesdorf, J., Xu, P., Pedersen, M. T., Clausen, M. P., Bonilla, J. C. (2025). Quantifying microscopic droplets in colloidal systems through machine learning-based image analysis. Food Hydrocolloids, 166, 111301. https://doi.org/10.1016/j.foodhyd.2025.111301
- Hu, Y.-T., Ting, Y., Hu, J.-Y., Hsieh, S.-C. (2017). Techniques and methods to study functional characteristics of emulsion systems. Journal of Food and Drug Analysis, 25 (1), 16–26. https://doi.org/10.1016/j.jfda.2016.10.021
- Tripathi, S., Bhattacharya, A., Singh, R., Tabor, R. F. (2017). Rheological behavior of high internal phase water-in-oil emulsions: Effects of droplet size, phase mass fractions, salt concentration and aging. Chemical Engineering Science, 174, 290–301. https://doi.org/10.1016/j.ces.2017.09.016
- Marín Castaño, E. P., Leite, R. H. T., de Souza Mendes, P. R. (2021). Microscopic phenomena inferred from the rheological analysis of an emulsion. Physics of Fluids, 33 (7). https://doi.org/10.1063/5.0053408
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Copyright (c) 2026 Volodymyr Kosenko, Anton Korotynskyi, Oleksandr Seminskyi

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