Identification of Enterobacter sakazakii in raw milk for dry infant formula production

Authors

DOI:

https://doi.org/10.15587/1729-4061.2014.23698

Keywords:

enterobactersakazakii, dry infant formulas, total microbial count, raw milk

Abstract

Microorganism E. sakazakii was first identified inUkrainein general and in raw cow’s milk in particular. Cultural, morphological and biochemical properties of 8 strains of this microorganism were studied, whereby one of the strains has been deposited with the National Collection. E. sakazakii was identified in raw milk in 22.3 % of cases. Direct correlation between the total bacterial milk pollution and E. sakazakii identification was determined. Average E. sakazakii count in 1 ml of raw milk, in which the total microbial count was up to 100.000 CFU/ml, was from 3 CFU/ml to 10 CFU/ml. In milk with high total bacterial pollution level − 500.000 CFU/ml, the E. sakazakii count was on average 150 CFU/ml − 200 CFU/ml. Optimal raw milk storage conditions:4 °Cfor 12−24 hours, in which the E. sakazakii growth and development slows down were determined. Thermoresistivity level of E. sakazakii, which is58 °C−60 °Cwas defined. It was proved that the raw cow’s milk can be a potential source of contamination of dry infant formulas by Enterobactersakazakii in case of failure of proper control over its obtaining, storage, transportation and processing and therefore pose a threat to children under the age of 12 months, who are fed by these formulas. It is recommended to pasteurize raw milk for producing dry infant formulas at a temperature no less than70 °C.

Author Biographies

Александра Николаевна Бергилевич, Sumy National Agrarian University Gerasima Kondraieva 160, Sumy, Ukraine, 40021

Doctor of Veterinary Sciences, Professor

Department of Food Technology (chair of Milk and MeatTechnology)

Евгения Анатольевна Гришина, Sumy National Agrarian University Gerasima Kondraieva 160, Sumy, Ukraine, 40021

PhD-student

Department of Food Technology (chair of Milk and MeatTechnology)

Виктория Викторовна Касянчук, Sumy National Agrarian University Gerasima Kondraieva 160, Sumy, Ukraine, 40021

Doctor of Veterinary Sciences, Professor

Department of Food Technology (chair of Milk and MeatTechnology)

References

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  24. Iversen, C., Lehner, A., Mullane, N. (2007). The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and description of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies. BMC Evol Biol., 7, 64–67.
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Published

2014-04-25

How to Cite

Бергилевич, А. Н., Гришина, Е. А., & Касянчук, В. В. (2014). Identification of Enterobacter sakazakii in raw milk for dry infant formula production. Eastern-European Journal of Enterprise Technologies, 2(12(68), 42–47. https://doi.org/10.15587/1729-4061.2014.23698

Issue

Vol. 2 No. 12(68) (2014): Technology and Equipment of Food Production

Section

Technology and Equipment of Food Production

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Copyright (c) 2014 Александра Николаевна Бергилевич, Евгения Анатольевна Гришина, Виктория Викторовна Касянчук

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