Establishing the dependence of fermentation process of milk clots with mesophilic lactic acid streptococci on the genotype of cows in terms of the kappa-casein gene

Authors

DOI:

https://doi.org/10.15587/1729-4061.2025.333997

Keywords:

kappa-casein, milk clot, lactic acid bacteria, consistency, viscosity, biotechnological processing, lactococci, technological properties, storage of fermented milk products

Abstract

The object of research is patterns in a technological process related to the fermentation of milk clots from dairy raw materials obtained from cows with different genotypes by the kappa-casein gene. The task still unresolved is the need to adjust the technological process in the manufacture of fermented milk products from dairy raw materials obtained from cows with different genotypes by this gene. This is associated with the interest of animal owners to create herds of cows with the BB genotype in order to improve milk suitability for cheese making.

 Changes in physical-chemical, microbiological, and organoleptic parameters in the process of targeted use of mesophilic lactic acid bacteria of biotechnological processing and during storage have been studied.

Based on the results of the study of fermented clots, their physical-chemical and microbiological parameters, the absence of dependence of the fermentation process of milk clots by mesophilic lactic acid streptococci on the cow genotype according to the kappa-casein gene has been found.

The study has resulted in confirming the hypothesis that genetic variants of the kappa-casein (CSN3) gene in cows (AA, AB, BB) affect the physical-chemical characteristics of milk, the dynamics of fermentation, and the properties of milk clots formed under the action of mesophilic lactic acid streptococci, and therefore the quality of finished fermented milk products. The use of milk from the cow genotype based on the kappa-casein gene is possible using classical technology and does not require adjustment of technological conditions.

The results of the work could be used in the milk processing industry in the development of technology for fermented milk products from raw milk of cows with different genotypes based on the kappa-casein gene

Author Biographies

Volodymyr Ladyka, Sumy National Agrarian University

Doctor of Agricultural Sciences, Professor, Academician of NAAS

Department of Technology of Production and Processing of Animal Products and Cinology

Yury Skliarenko, Institute of Agriculture of the Northeast of the National Academy of Sciences

Doctor of Agricultural Sciences

Laboratory of Animal Husbandry and Fodder Production

Nataliia Bolgova, Sumy National Agrarian University

PhD, Associate Professor

Department of Technology and Food Safety

Viktoriia Vechorka, Khmelnytskyi National University

Doctor of Agricultural Sciences, Professor

Department of Industrial Engineering and Agroengineering

Serhiy Tereshchenko, Sumy National Agrarian University

PhD Student

Department of Animal Genetics, Breeding and Biotechnology

Vitalii Pysariev, Sumy National Agrarian University

PhD Student

Department of Animal Genetics, Breeding and Biotechnology

References

  1. Sakihara, T., Otsuji, K., Arakaki, Y., Hamada, K., Sugiura, S., Ito, K. (2022). Early Discontinuation of Cow’s Milk Protein Ingestion Is Associated with the Development of Cow’s Milk Allergy. The Journal of Allergy and Clinical Immunology: In Practice, 10 (1), 172–179. https://doi.org/10.1016/j.jaip.2021.07.053
  2. Giannetti, A., Toschi Vespasiani, G., Ricci, G., Miniaci, A., di Palmo, E., Pession, A. (2021). Cow’s Milk Protein Allergy as a Model of Food Allergies. Nutrients, 13 (5), 1525. https://doi.org/10.3390/nu13051525
  3. Čítek, J., Brzáková, M., Hanusová, L., Hanuš, O., Večerek, L., Samková, E. et al. (2020). Technological properties of cow’s milk: correlations with milk composition, effect of interactions of genes and other factors. Czech Journal of Animal Science, 65 (1), 13–22. https://doi.org/10.17221/150/2019-cjas
  4. Sokoliuk, V. M., Dukhnytsky, V. B., Krupelnytsky, T. V., Ligomina, I. P., Revunets, A. S., Prus, V. M. (2022). Influence of technological factors on milk quality indicators. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 24 (105), 37–43. https://doi.org/10.32718/nvlvet10506
  5. Giribaldi, M., Lamberti, C., Cirrincione, S., Giuffrida, M. G., Cavallarin, L. (2022). A2 Milk and BCM-7 Peptide as Emerging Parameters of Milk Quality. Frontiers in Nutrition, 9. https://doi.org/10.3389/fnut.2022.842375
  6. Plivachuk, О. P., Dyman, T. M. (2018). Interdependence of complex genotypes of alfa-lactalbumin and beta-lactoglobulin with composition and technological properties of milk of ukrainian black-and-white dairy cattle. Animal Breeding and Genetics, 51, 124–131. https://doi.org/10.31073/abg.51.17
  7. Manguy, J., Shields, D. C. (2019). Implications of kappa-casein evolutionary diversity for the self-assembly and aggregation of casein micelles. Royal Society Open Science, 6 (10), 190939. https://doi.org/10.1098/rsos.190939
  8. Khastayeva, A. Zh., Mamayeva, L. A., Abylgazinova, A. T., Zhamurova, V. S., Karimov, N. Zh., Muratbekova, K. M. (2021). RETRACTED ARTICLE: Influence of the kappa casein genotype on the technological properties of cow milk of Simmental and Alatau breeds. Functional & Integrative Genomics, 21 (2), 231–238. https://doi.org/10.1007/s10142-021-00772-1
  9. Bonfatti, V., Di Martino, G., Cecchinato, A., Vicario, D., Carnier, P. (2010). Effects of β-κ-casein (CSN2-CSN3) haplotypes and β-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows. Journal of Dairy Science, 93 (8), 3797–3808. https://doi.org/10.3168/jds.2009-2778
  10. Bonfatti, V., Chiarot, G., Carnier, P. (2014). Glycosylation of κ-casein: Genetic and nongenetic variation and effects on rennet coagulation properties of milk. Journal of Dairy Science, 97 (4), 1961–1969. https://doi.org/10.3168/jds.2013-7418
  11. Kyselová, J., Ječmínková, K., Matějíčková, J., Hanuš, O., Kott, T., Štípková, M., Krejčová, M. (2019). Physiochemical characteristics and fermentation ability of milk from Czech Fleckvieh cows are related to genetic polymorphisms of β-casein, κ-casein, and β-lactoglobulin. Asian-Australasian Journal of Animal Sciences, 32 (1), 14–22. https://doi.org/10.5713/ajas.17.0924
  12. Abeykoon, C. D., Rathnayake, R. M. C., Johansson, M., Silva, G. L. L. P., Ranadheera, C. S., Lundh, Å., Vidanarachchi, J. K. (2016). Milk Coagulation Properties and Milk Protein Genetic Variants of Three Cattle Breeds/Types in Sri Lanka. Procedia Food Science, 6, 348–351. https://doi.org/10.1016/j.profoo.2016.02.070
  13. Gustavsson, F., Buitenhuis, A. J., Johansson, M., Bertelsen, H. P., Glantz, M., Poulsen, N. A. et al. (2014). Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. Journal of Dairy Science, 97 (6), 3866–3877. https://doi.org/10.3168/jds.2013-7312
  14. Bijl, E., van Valenberg, H., Sikkes, S., Jumelet, S., Sala, G., Olieman, K. et al. (2014). Chymosin-induced hydrolysis of caseins: Influence of degree of phosphorylation of alpha-s1-casein and genetic variants of beta-casein. International Dairy Journal, 39 (2), 215–221. https://doi.org/10.1016/j.idairyj.2014.07.005
  15. Sun, Y., Ding, Y., Liu, B., Guo, J., Su, Y., Yang, X. et al. (2024). Recent advances in the bovine β-casein gene mutants on functional characteristics and nutritional health of dairy products: Status, challenges, and prospects. Food Chemistry, 443, 138510. https://doi.org/10.1016/j.foodchem.2024.138510
  16. Bolgova, N. V., Huba, S. O., Sokolenko, V. V., Mazhara, A. K. (2023). Study of the influence of vitamins on the fermentation process in the production of yogurt. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 25 (100), 43–46. https://doi.org/10.32718/nvlvet-f10007
  17. Bintsis, T., Papademas, P. (2022). The Evolution of Fermented Milks, from Artisanal to Industrial Products: A Critical Review. Fermentation, 8 (12), 679. https://doi.org/10.3390/fermentation8120679
  18. Samilyk, M., Bolgova, N., Samokhina, E., Cherniavska, T., Kharchenko, S. (2024). Use of hop extract in the biotechnology of kefir beverage. Scientific Horizons, 27 (3), 97–106. https://doi.org/10.48077/scihor3.2024.97
  19. Wang, X., Yu, Z., Zhao, X., Han, R., Huang, D., Yang, Y., Cheng, G. (2020). Comparative proteomic characterization of bovine milk containing β‐casein variants A1A1 and A2A2, and their heterozygote A1A2. Journal of the Science of Food and Agriculture, 101 (2), 718–725. https://doi.org/10.1002/jsfa.10684
  20. Liu, T., Li, Y., Yang, Y., Yi, H., Zhang, L., He, G. (2020). The influence of different lactic acid bacteria on sourdough flavor and a deep insight into sourdough fermentation through RNA sequencing. Food Chemistry, 307, 125529. https://doi.org/10.1016/j.foodchem.2019.125529
  21. Romanchuk, I., Minorova, A., Rudakova, T., Moiseeva, L. (2020). Regularities of lactose hydrolysis in dairy raw materials. Food Resources, 8 (14), 165–174. Available at: https://iprjournal.kyiv.ua/index.php/pr/article/view/55
  22. Gao, D., Helikh, A., Duan, Z. (2021). Functional properties of four kinds of oilseed protein isolates. Journal of Chemistry and Technologies, 29 (1), 155–163. https://doi.org/10.15421/082116
  23. Kieliszek, M., Pobiega, K., Piwowarek, K., Kot, A. M. (2021). Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria. Molecules, 26 (7), 1858. https://doi.org/10.3390/molecules26071858
  24. Cheng, T., Wang, L., Guo, Z., Li, B. (2022). Technological characterization and antibacterial activity of Lactococcus lactis subsp. cremoris strains for potential use as starter culture for cheddar cheese manufacture. Food Science and Technology, 42. https://doi.org/10.1590/fst.13022
  25. Thum, C., Roy, N. C., Everett, D. W., McNabb, W. C. (2021). Variation in milk fat globule size and composition: A source of bioactives for human health. Critical Reviews in Food Science and Nutrition, 63 (1), 87–113. https://doi.org/10.1080/10408398.2021.1944049
  26. Ladyka, V., Drevytska, T., Pavlenko, J., Skliarenko, Y., Lahuta, T., Drevytskyi, O., Dosenko, V. (2022). Evaluation of cow genotypes by kappa-casein of dairy breeds. Acta Fytotechnica et Zootechnica, 25 (1). https://doi.org/10.15414/afz.2022.25.01.1-6
  27. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes Text with EEA relevance. Available at: https://eur-lex.europa.eu/eli/dir/2010/63/oj/eng
  28. Nakaz No. 249 vid 01.03.2012 Pro zatverdzhennia Poriadku provedennia naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh. Available at: https://zakononline.com.ua/documents/show/329995___330060
  29. Teliuk, P. A. (2021). Vplyv natriu, kaliu ta kaltsiu na rozvytok Lactococcus lactis. Kyiv. Available at: https://er.nau.edu.ua/items/4e03963d-1bb0-44f0-a01c-d9fa0b65c93f
  30. Miluchová, M., Gábor, M., Candrák, J., Trakovická, A., Candráková, K. (2018). Association of HindIII-polymorphism in kappa-casein gene with milk, fat and protein yield in holstein cattle*. Acta Biochimica Polonica, 65 (3). https://doi.org/10.18388/abp.2017_2313
Establishing the dependence of fermentation process of milk clots with mesophilic lactic acid streptococci on the genotype of cows in terms of the kappa-casein gene

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Published

2025-08-26

How to Cite

Ladyka, V., Skliarenko, Y., Bolgova, N., Vechorka, V., Tereshchenko, S., & Pysariev, V. (2025). Establishing the dependence of fermentation process of milk clots with mesophilic lactic acid streptococci on the genotype of cows in terms of the kappa-casein gene . Eastern-European Journal of Enterprise Technologies, 4(11 (136), 49–56. https://doi.org/10.15587/1729-4061.2025.333997

Issue

Vol. 4 No. 11 (136) (2025): Technology and Equipment of Food Production

Section

Technology and Equipment of Food Production

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Copyright (c) 2025 Volodymyr Ladyka, Yury Skliarenko, Nataliia Bolhova, Viktoriia Vechorka, Serhiy Tereshchenko, Vitalii Pysariev

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